Publication: Bioconversion of Napier grass to 2-phenylethanol: unveiling the synergy of pretreatment with deep eutectic solvents and yeasts
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Issued Date
2024-01-01
Resource Type
ISSN
17272394
eISSN
17277841
Scopus ID
2-s2.0-85216521412
Journal Title
International Journal of Applied Science and Engineering
Volume
21
Issue
5
Start Page
1
End Page
13
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Applied Science and Engineering Vol.21 No.5 (2024) , 1-13
Suggested Citation
Panakkal E.J., Cheng Y.S., Khoo K.S., Show P.L., Tantayotai P., Sriariyanun M. Bioconversion of Napier grass to 2-phenylethanol: unveiling the synergy of pretreatment with deep eutectic solvents and yeasts. International Journal of Applied Science and Engineering Vol.21 No.5 (2024) , 1-13. 13. doi:10.6703/IJASE.202412_21(5).006 Retrieved from: https://hdl.handle.net/20.500.14740/20874
Corresponding Author(s)
Other Contributor(s)
Abstract
2-Phenylethanol (2-PE), a valuable flavor compound with high market demand, can be produced from readily available lignocellulosic biomass. This study explored the potential of Napier grass, a fast-growing energy crop, as a substrate for 2-PE production. Napier grass was pretreated using three different deep eutectic solvents (DES) formulated with choline chloride (ChCl) combined with lactic acid (LA), glycerol (Gly), or urea (Urea). DES pretreatment partially removed hemicellulose and increased cellulose content (up to 40%) in the biomass. Subsequent enzymatic hydrolysis revealed the highest glucose yield, from ChCl/LA pretreated biomass, almost 25% more than the native biomass. Fermentation optimization for temperature and nitrogen content identified strain-specific optimal conditions for 2-PE and ethanol production using two non-conventional yeasts, Kluyveromyces marxianus and Pichia kudriavzevii. Notably, ChCl/Gly pretreated biomass with P. kudriavzevii produced the highest 2-PE yield (510.51 ppm) alongside significant ethanol production (30.63 g/L). This study paves the way for a more sustainable approach to producing 2-PE, a valuable flavor compound, using readily available biomass and non-conventional yeasts.