Publication: Pectin nanoparticle enhances cytotoxicity of methotrexate against hepG2 cells
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
10717544
Other identifier(s)
2-s2.0-84872251182
Rights Holder(s)
Scopus
Bibliographic Citation
Drug Delivery. Vol 20, No.1 (2013), p.1-9
Suggested Citation
Chittasupho C., Jaturanpinyo M., Mangmool S. Pectin nanoparticle enhances cytotoxicity of methotrexate against hepG2 cells. Drug Delivery. Vol 20, No.1 (2013), p.1-9. doi:10.3109/10717544.2012.739214 Retrieved from: https://hdl.handle.net/20.500.14740/6728
Author(s)
Abstract
Objective: This work has aimed to develop methotrexate-conjugated pectin nanoparticle for delivering a cytotoxic drug to hepatic cancer cell. Methods: Methotrexate was conjugated to pectin by carbodiimide chemistry. Nanoparticles of pectin conjugated with methotrexate (MTX) were then fabricated by using ionotropic gelation. The size, shape and surface charge were characterized by dynamic light scattering and microscopic method. Cytotoxicity of MTX-pectin nanoparticle was monitored by MTT assay. Results: Methotrexate-pectin nanoparticle was successfully formulated. Drug release study indicated that MTX-NP exhibited sustained drug release at pH 7.4. Sustained release of methotrexate may enable methotrexate-pectin nanoparticle as a controlled drug delivery system. Cytotoxicity study confirmed the activity of the drug incorporated in nanoparticles. In addition, the cytotoxicity of methotrexate was increased when conjugated to pectin nanoparticles, compared to free methotrexate. Conclusions: This study verified that pectin can deliver methotrexate to hepatic cancer cell and provide sustained drug delivery. The cytotoxicity of methotrexate was enhanced when methotrexate was conjugated to pectin indicating the improved drug delivery to cancer cell. © 2013 Informa Healthcare USA, Inc.
Subject(s)
Methotrexate
Nanoparticle
Pectin
Article
Cancer cell culture
Cytotoxicity
Drug delivery system
Drug release
Drug structure
Human
Human cell
In vitro study
Liver cancer
PH measurement
Priority journal
Antimetabolites, Antineoplastic
Cytotoxins
Drug Delivery Systems
Hep G2 Cells
Humans
Methotrexate
Nanoparticles
Pectins
Nanoparticle
Pectin
Article
Cancer cell culture
Cytotoxicity
Drug delivery system
Drug release
Drug structure
Human
Human cell
In vitro study
Liver cancer
PH measurement
Priority journal
Antimetabolites, Antineoplastic
Cytotoxins
Drug Delivery Systems
Hep G2 Cells
Humans
Methotrexate
Nanoparticles
Pectins