Publication:
Development of monoclonal antibodies for simple identification of Vibrio alginolyticus

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

2668254

DOI

10.1111/j.1472-765X.2006.01969.x

Other identifier(s)

2-s2.0-33748617109

Rights Holder(s)

Scopus

Bibliographic Citation

Letters in Applied Microbiology. Vol 43, No.4 (2006), p.436-442

Suggested Citation

Sithigorngul W., Rengpipat S., Tansirisittikul A., Rukpratanporn S., Longyant S., Chaivisuthangkura P., Sithigorngul P. Development of monoclonal antibodies for simple identification of Vibrio alginolyticus. Letters in Applied Microbiology. Vol 43, No.4 (2006), p.436-442. doi:10.1111/j.1472-765X.2006.01969.x Retrieved from: https://hdl.handle.net/20.500.14740/5422

Title

Development of monoclonal antibodies for simple identification of Vibrio alginolyticus

Author(s)

Sithigorngul W.
 Rengpipat S.
 Tansirisittikul A.
 Rukpratanporn S.
 Longyant S.
 Chaivisuthangkura P.
 Sithigorngul P.

Abstract

Aims: The present study was aimed to produce monoclonal antibodies (MAbs) for simple and specific identification of Vibrio alginolyticus infection in shrimp. Methods and Results: Mice were immunized with heat killed V. alginolyticus four times at 2-week intervals. The best response mouse was used for spleen donor in hybridoma production. Screening of hybridoma clones producing desired antibodies was performed by dot blotting against V. alginolyticus and other bacterial species, Western blotting and immunohistochemistry of infected shrimp tissues. Four groups of MAbs were obtained; the first group of MAbs demonstrated their limited specificity only to V. alginolyticus used for immunization, while the second and the third groups recognized all three isolates of V. alginolyticus used for testing. The fourth group of MAbs bound to all three isolates of V. alginolyticus and also recognized Vibrio parahaemolyticus, Vibrio harveyi, Vibrio fluvialis and Vibrio vulnificus but did not bind to Vibrio mimicus, Vibrio cholerae, Vibrio penaeicida and other bacterial species tested. MAbs in groups 1, 2 and 3 were able to use for the detection of bacterial infection in the tissues by means of immunohistochemistry. Conclusions: MAbs specific to V. alginolyticus was produced. These MAbs can be used for specific identification of the bacteria by simple 'dot blotting' method and immunohistochemistry. Significance and Impact of the Study: This study demonstrated an immunological tool that can be used for simple and accurate identification of V. alginolyticus as well as for the diagnosis of V. alginolyticus infection in animals. This immunological tool can replace costly and laborious biochemical tests. © 2006 The Authors.

Subject(s)

Monoclonal antibody
 Antibodies
 Bacteria
 Cloning
 Diseases
 Immunology
 Antibody
 Bacterial disease
 Bacterium
 Crustacean
 Identification method
 Immunization
 Immunoassay
 Rodent
 Animal tissue
 Article
 Bacterium identification
 Controlled study
 Gram negative infection
 Immunohistochemistry
 Mouse
 Nonhuman
 Shrimp
 Vibrio alginolyticus
 Vibrio cholerae
 Vibrio harveyi
 Vibrio mimicus
 Vibrio parahaemolyticus
 Vibrio vulnificus
 Animals
 Antibodies, Monoclonal
 Antibody Specificity
 Blotting, Western
 Immunohistochemistry
 Mice
 Shellfish
 Vibrio alginolyticus
 Vibrio Infections
 Animalia
 Bacteria (microorganisms)
 Decapoda (Crustacea)
 Penaeus monodon
 Vibrio alginolyticus
 Vibrio cholerae
 Vibrio fluvialis
 Vibrio harveyi
 Vibrio mimicus
 Vibrio parahaemolyticus
 Vibrio penaeicida
 Vibrio vulnificus

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URI

https://hdl.handle.net/20.500.14740/5422

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Scopus: Year 1983-2021

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