Publication:
Endogenous rhythmicity of Bmal1 and Rev-erbα in the hamster pineal gland is not driven by norepinephrine

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Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

0953816X

DOI

10.1111/j.1460-9568.2009.06742.x

Other identifier(s)

2-s2.0-65749084837

Rights Holder(s)

Scopus

Bibliographic Citation

European Journal of Neuroscience. Vol 29, No.10 (2009), p.2009-2016

Suggested Citation

Wongchitrat P., Felder-Schmittbuhl M.-P., Phansuwan-Pujito P., Pévet P., Simonneaux V. Endogenous rhythmicity of Bmal1 and Rev-erbα in the hamster pineal gland is not driven by norepinephrine. European Journal of Neuroscience. Vol 29, No.10 (2009), p.2009-2016. doi:10.1111/j.1460-9568.2009.06742.x Retrieved from: https://hdl.handle.net/20.500.14740/6857

Title

Endogenous rhythmicity of Bmal1 and Rev-erbα in the hamster pineal gland is not driven by norepinephrine

Author(s)

Wongchitrat P.
 Felder-Schmittbuhl M.-P.
 Phansuwan-Pujito P.
 Pévet P.
 Simonneaux V.

Abstract

Pineal melatonin is synthesized with daily and seasonal rhythms following the hypothalamic clock-driven release of norepinephrine (NE). The pineal gland of rats and mice, like the biological clock, expresses a number of clock genes. However, the role of pineal clock elements in pineal physiology is still unknown. We examined the expression and regulation of several clock genes (Per1, Cry2, Bmal1 and Rev-erbα) under different lighting conditions or following adrenergic treatments in the Syrian hamster, a seasonal rodent. We found that Per1 and Cry2 genes were similarly regulated by the nocturnal release of NE: levels of Per1 and Cry2 mRNA displayed a nocturnal increase that was maintained after 2 days in constant darkness (DD) but abolished after 2 days under constant light (LL), a condition that suppresses endogenous NE release, or after an early night administration of the adrenergic antagonist propranolol. In contrast, Bmal1 and Rev-erbα exhibited a different pattern of expression and regulation. mRNA levels of both clock genes displayed a marked daily variation, maintained in DD, with higher values at midday for Bmal1 and at day/night transition for Rev-erbα. Remarkably, the daily variation of both Bmal1 and Rev-erbα mRNA was maintained in LL conditions and was not affected by propranolol. This study confirms the daily regulation of Per1 and Cry2 gene expression by NE in the pineal gland of rodents and shows for the first time that a second set of clock genes, Bmal1 and Rev-erbα are expressed with a circadian rhythm independent of the hypothalamic clock-driven noradrenergic signal. © Federation of European Neuroscience Societies and Blackwell Publishing Ltd.

Subject(s)

Cryptochrome 2
 Messenger RNA
 Noradrenalin
 PER1 protein
 Propranolol
 Protein BMAL1
 Rev erb alpha protein
 Transcription factor CLOCK
 Unclassified drug
 Animal experiment
 Animal tissue
 Article
 Circadian rhythm
 Controlled study
 Female
 Gene expression profiling
 Gene expression regulation
 Genetic variability
 Hypothalamus
 Light dark cycle
 Nonhuman
 Noradrenalin release
 Noradrenergic system
 Pineal body
 Priority journal
 Protein content
 Signal transduction
 Syrian hamster
 Animals
 Basic Helix-Loop-Helix Transcription Factors
 Biological Clocks
 Circadian Rhythm
 Cricetinae
 Female
 Gene Expression
 Gene Expression Regulation
 Mesocricetus
 Norepinephrine
 Pineal Gland

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URI

https://hdl.handle.net/20.500.14740/6857

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Scopus: Year 1983-2021

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