Publication: Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD)
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
3044017
Other identifier(s)
2-s2.0-85102122541
Rights Holder(s)
Scopus
Bibliographic Citation
Veterinary Parasitology. Vol 292, No. (2021)
Suggested Citation
Panich W., Tejangkura T., Chontananarth T. Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD). Veterinary Parasitology. Vol 292, No. (2021). doi:10.1016/j.vetpar.2021.109396 Retrieved from: https://hdl.handle.net/20.500.14740/7846
Author(s)
Abstract
Cestodes belonging to the genus Raillietina are a major veterinary health problem in the poultry industry, especially in chickens (Gallus gallus domesticus) and ducks (Anas playtrhynchos domesticus). In this study, loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay was established and validated for the detection of R. echinobothrida, R. tetragona, and R. cesticillus in one reaction. The LAMP-LFD assay can be completed in 75 min under isothermal conditions at 66 °C and the results can be obtained by observation with the naked eye. This assay was very specific and had no cross-amplification with other closely related parasites (Cotugnia sp., Diorchis formosensis, Fimbriaria sp., Echinostoma sp., E. miyagawai, Hypoderaeum conoideum, Prosthogonimus cuneatus, and Ascaridia galli) or their definitive hosts (G. g. domesticus, A. p. domesticus). The sensitivity of the LAMP-LFD assay was detected with three Raillietina species at 0.5 ng, which was enough for gravid proglottid DNA detection. The accuracy test showed that the LAMP-LFD assay demonstrated accurate verification results when compared to morphological results. This is a novel LAMP-LFD assay that is highly specific and sensitive for the detection of Raillietina species. It can be applied to detection for epidemiological investigations, monitoring programs, surveillance, control, and to solve veterinary health problems for the poultry industry in Raillietina endemic areas. © 2021 Elsevier B.V.
Subject(s)
Article
Ascaridia galli
Controlled study
Echinostoma
Loop mediated isothermal amplification
Nonhuman
Parasite
Poultry
Veterinary medicine
Animal
Cestode
Classification
Genetics
Isolation and purification
Molecular diagnosis
Nucleic acid amplification
Procedures
Species difference
Helminth DNA
Helminth RNA
RNA 28S
Animals
Cestoda
DNA, Helminth
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
RNA, Helminth
RNA, Ribosomal, 28S
Species Specificity
Ascaridia galli
Controlled study
Echinostoma
Loop mediated isothermal amplification
Nonhuman
Parasite
Poultry
Veterinary medicine
Animal
Cestode
Classification
Genetics
Isolation and purification
Molecular diagnosis
Nucleic acid amplification
Procedures
Species difference
Helminth DNA
Helminth RNA
RNA 28S
Animals
Cestoda
DNA, Helminth
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
RNA, Helminth
RNA, Ribosomal, 28S
Species Specificity