Publication:
Effect of pre-soaking mustard green seeds prior to cold plasma treatment on bioactive aspects of microgreens

0
0

Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

26160684

DOI

10.26538/tjnpr/v5i8.16

Other identifier(s)

2-s2.0-85116155264

Rights Holder(s)

Scopus

Bibliographic Citation

Tropical Journal of Natural Product Research. Vol 5, No.8 (2021), p.1422-1426

Suggested Citation

Matra K., Saengha W., Tanakaran Y., Karirat T., Buranrat B., Deeseenthum S., Luang-In V. Effect of pre-soaking mustard green seeds prior to cold plasma treatment on bioactive aspects of microgreens. Tropical Journal of Natural Product Research. Vol 5, No.8 (2021), p.1422-1426. doi:10.26538/tjnpr/v5i8.16 Retrieved from: https://hdl.handle.net/20.500.14740/5132

Title

Effect of pre-soaking mustard green seeds prior to cold plasma treatment on bioactive aspects of microgreens

Author(s)

Matra K.
 Saengha W.
 Tanakaran Y.
 Karirat T.
 Buranrat B.
 Deeseenthum S.
 Luang-In V.

Abstract

Microgreens have been reported to contain higher phytochemicals than mature plants. Mustard green (MG) is rich in glucosinolates that produce isothiocyanates (ITCs) upon degradation. ITCs have well-known chemopreventive benefits. Previously, non-thermal plasma treatment on MG seeds could enhance bioactive content; However, seed pretreatment before non-thermal plasma treatment was yet to be studied. The aim of this work was to evaluate the impact of pre-soaking MG seeds overnight before non-thermal plasma treatment at 21 kV for 5 min on phytochemicals and activities of MG microgreens. Plasma-treated pre-soaked (PTPS) seeds and control seeds (no plasma treatment) received deionized water for 7 days. No difference in germination percentage, stem height or fresh/dried weight of MG between PTPS seeds and the control was observed. Total ITC content in MG microgreens from PTPS seeds (1.62 mmol/100 g DW) was significantly higher than control seeds (1.00 mmol/100 g DW). Antioxidant capacity and total phenolic/flavonoid compounds were similar in both MG microgreens. Lower IC50 values were recorded for MG microgreens from PTPS seeds at most time intervals, indicating higher cytotoxicity against MCF-7 and HepG2, and greater effectiveness in cancer proliferation inhibition than the control. No differences in Bax, Caspase-3 and Bcl-2 mRNA expressions were detected for all samples; however, MMP-9 mRNA expression was reduced in MG microgreens from PTPS seeds for HepG2. Pre-soaking MG seeds before plasma treatment at 21 kV for 5 min increased ITC levels and increased anticancer activities of MG microgreens without adverse effects compared to MG microgreens from control seeds. © 2021 Matra et al.

Subject(s)

Antioxidant
 Caspase 3
 Deionized water
 Flavonoid
 Gallic acid
 Glucosinolate
 Isothiocyanic acid
 Isothiocyanic acid derivative
 Linoleic acid
 Magnesium sulfate
 Messenger RNA
 Mustard green seed extract
 Phytochemical
 Plant extract
 Polyphenol
 Protein Bax
 Reactive oxygen metabolite
 Unclassified drug
 Antineoplastic activity
 Antioxidant activity
 Apoptosis
 Article
 Cancer growth
 Cell migration
 Cell proliferation
 Cold plasma treatment
 Cold stress
 Colony formation
 Controlled study
 Cytotoxicity
 DNA fragmentation
 DPPH radical scavenging assay
 Dry weight
 Extraction
 Ferric reducing antioxidant power assay
 Gene expression
 Germination
 Human
 Human cell
 Human tissue
 IC50
 Investigative procedures
 MTT assay
 Mustard
 Nonhuman
 Plasma gas
 Polymerase chain reaction
 Protein expression
 RNA extraction
 RNA isolation
 Soaking
 Stem elongation
 Western blotting
 Wound healing
 Wound healing assay

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/5132

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By