Publication: Simple and direct detection of Aeromonas hydrophila infection in the goldfish, Carassius auratus (L.), by dot blotting using specific monoclonal antibodies
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0
Issued Date
2010
Resource Type
File Type
application/pdf
ISSN
1407775
Other identifier(s)
2-s2.0-78649486209
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of Fish Diseases. Vol 33, No.12 (2010), p.973-984
Suggested Citation
Longyant S., Chaiyasittrakul K., Rukpratanporn S., Chaivisuthangkura P., Sithigorngul P. Simple and direct detection of Aeromonas hydrophila infection in the goldfish, Carassius auratus (L.), by dot blotting using specific monoclonal antibodies. Journal of Fish Diseases. Vol 33, No.12 (2010), p.973-984. doi:10.1111/j.1365-2761.2010.01197.x Retrieved from: https://hdl.handle.net/20.500.14740/7479
Abstract
A combination of eight isolates of Aeromonas hydrophila was used to produce monoclonal antibodies (MAbs). Ten different groups of MAbs specific to Aeromonas were selected. The first five groups of MAbs demonstrated high specificity and bound to only one or two isolates of A. hydrophila. The sixth and the seventh groups of MAbs were A. hydrophila specific. They recognized seven of eight A. hydrophila isolates (AH1, 2, 3, 4, 5, 6, 8); however, the MAb in the seventh group also showed cross-reactivity to one isolate of Aeromonas caviae (AC3). The eighth MAb group recognized two isolates of A. hydrophila (AH2 and AH5) and demonstrated cross-reactivity to one isolate of Aeromonas sobria (AS1) and one isolate of A. caviae (AC3). The tenth group of MAbs bound to all isolates of Aeromonas spp. tested (AH1-8, AS1-6, AC1-5, Aeromonas veronii and Aeromonas jandaei) without cross-reactivity to any of the other bacteria tested. MAbs in the ninth group showed similar specificity to those in the tenth group but did not recognize two isolates of A. sobria (AS4 and AS6) or A. jandaei. All the MAbs could be used to identify Aeromonas by dot blotting with a sensitivity ranging from 105 to 107 CFU mL-1. However, the sensitivity of detection was increased to 102-103 CFU mL-1 after inoculation of the sample in tryptic soy broth for 3-6 h before performing the dot blotting. The dot blot method can be used for the direct detection of A. hydrophila infection in symptomatic and asymptomatic goldfish. This study demonstrated a convenient immunological tool that can be used for the direct detection of A. hydrophila and Aeromonas infections in a complex sample without the requirement for separation of the bacteria or isolation and biochemical tests. © 2010 Blackwell Publishing Ltd.
Subject(s)
Bacterium antibody
Diagnostic agent
Monoclonal antibody
Aeromonas hydrophila
Animal
Animal disease
Article
Fish disease
Goldfish
Gram negative infection
Immunoblotting
Methodology
Microbiology
Physiology
Sensitivity and specificity
Aeromonas hydrophila
Animals
Antibodies, Bacterial
Antibodies, Monoclonal
Fish Diseases
Goldfish
Gram-Negative Bacterial Infections
Immunoblotting
Sensitivity and Specificity
Aeromonas
Aeromonas hydrophila
Aeromonas jandaei
Aeromonas punctata
Aeromonas sobria
Aeromonas veronii
Carassius auratus
Diagnostic agent
Monoclonal antibody
Aeromonas hydrophila
Animal
Animal disease
Article
Fish disease
Goldfish
Gram negative infection
Immunoblotting
Methodology
Microbiology
Physiology
Sensitivity and specificity
Aeromonas hydrophila
Animals
Antibodies, Bacterial
Antibodies, Monoclonal
Fish Diseases
Goldfish
Gram-Negative Bacterial Infections
Immunoblotting
Sensitivity and Specificity
Aeromonas
Aeromonas hydrophila
Aeromonas jandaei
Aeromonas punctata
Aeromonas sobria
Aeromonas veronii
Carassius auratus