Publication:
Aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

9593993

DOI

10.1007/s11274-014-1639-7

Other identifier(s)

2-s2.0-84903527305

Rights Holder(s)

Scopus

Bibliographic Citation

World Journal of Microbiology and Biotechnology. Vol 30, No.8 (2014), p.2199-2204

Suggested Citation

Manitchotpisit P., Watanapoksin R., Price N.P.J., Bischoff K.M., Tayeh M., Teeraworawit S., Kriwong S., Leathers T.D. Aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity. World Journal of Microbiology and Biotechnology. Vol 30, No.8 (2014), p.2199-2204. doi:10.1007/s11274-014-1639-7 Retrieved from: https://hdl.handle.net/20.500.14740/6260

Title

Aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity

Author(s)

Manitchotpisit P.
 Watanapoksin R.
 Price N.P.J.
 Bischoff K.M.
 Tayeh M.
 Teeraworawit S.
 Kriwong S.
 Leathers T.D.

Abstract

Liamocins are structurally unique, heavier-than-water "oils" produced by certain strains of Aureobasidium pullulans. The aim of the current study is to identify new sources of liamocins and evaluate their potential as anticancer agents. Nine strains of A. pullulans from phylogenetic clades 8, 9, and 11 were examined for the first time for production of liamocins. Strains in these clades have only been isolated from tropical environments, and all strains tested here were from various locations in Thailand. Strains RSU 9, RSU 21, and RSU 29, all from clade 11, produced from 7.0 to 8.6 g liamocins/l from medium containing 5 % sucrose. These are the highest yields of liamocins that we have found thus far. These strains also produced from 9.4 to 17 g pullulan/l. The structural identity of liamocins was confirmed by matrix-assisted laser desorption/ionization mass spectrometry; differential spectra were obtained in which the dominant ion was either at about m/z 805.5 or m/z 949.6, consistent with the structure of liamocins. Liamocins from A. pullulans strains RSU 9 and RSU 21 inhibited two human breast cancer cell lines and a human cervical cancer cell line (IC 50 values of 32.2 ± 1.4 to 63.1 ± 2.4 μg liamocins/ml) but were not toxic to a normal cell line. Liamocins weakly inhibited a strain of Enterococcus faecalis, but did not inhibit strains of Lactobacillus fermentum, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Thus, A. pullulans phylogenetic clade 11 is a promising source of liamocins, and these compounds merit further examination as potential anticancer agents. © 2014 Springer Science+Business Media Dordrecht (outside the USA).

Subject(s)

Cell culture
 Cells
 Crude oil
 Desorption
 Diseases
 Escherichia coli
 Mass spectrometry
 Anti-cancer agents
 Aureobasidium pullulans
 Liamocins
 Phylogeny
 Pullulans
 Heavy oil production
 Aureobasidium pullulans
 Enterococcus faecalis
 Escherichia coli
 Lactobacillus fermentum
 Pseudomonas aeruginosa
 Staphylococcus aureus
 Antiinfective agent
 Antineoplastic agent
 Biological product
 Liamocin A1
 Liamocin B1
 Mannitol
 Oil
 Sugar alcohol
 Analogs and derivatives
 Animal
 Ascomycetes
 Bacteria
 Cell proliferation
 Chemical structure
 Chemistry
 Chlorocebus aethiops
 Classification
 Drug effects
 HeLa cell line
 Human
 Metabolism
 Neoplasms
 Pathology
 Tumor cell line
 Vero cell line
 Animals
 Anti-Bacterial Agents
 Antineoplastic Agents
 Ascomycota
 Bacteria
 Biological Products
 Cell Line, Tumor
 Cell Proliferation
 Cercopithecus aethiops
 HeLa Cells
 Humans
 Mannitol
 Molecular Structure
 Neoplasms
 Oils
 Sugar Alcohols
 Vero Cells

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URI

https://hdl.handle.net/20.500.14740/6260

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Scopus: Year 1983-2021

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