Publication:
Domain 2 of a Kazal serine proteinase inhibitor SPIPm2 from Penaeus monodon possesses antiviral activity against WSSV

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2014.09.036

Other identifier(s)

2-s2.0-84908397531

Rights Holder(s)

Scopus

Bibliographic Citation

Fish and Shellfish Immunology. Vol 41, No.2 (2014), p.526-530

Suggested Citation

Visetnan S., Donpudsa S., Supungul P., Tassanakajon A., Rimphanitchayakit V. Domain 2 of a Kazal serine proteinase inhibitor SPIPm2 from Penaeus monodon possesses antiviral activity against WSSV. Fish and Shellfish Immunology. Vol 41, No.2 (2014), p.526-530. doi:10.1016/j.fsi.2014.09.036 Retrieved from: https://hdl.handle.net/20.500.14740/6272

Title

Domain 2 of a Kazal serine proteinase inhibitor SPIPm2 from Penaeus monodon possesses antiviral activity against WSSV

Author(s)

Visetnan S.
 Donpudsa S.
 Supungul P.
 Tassanakajon A.
 Rimphanitchayakit V.

Abstract

A 5-domain Kazal type serine proteinase inhibitor SPI. Pm2 from Penaeus monodon is involved in innate immune defense against white spot syndrome virus (WSSV). To test which domains were involved, the 5 domains of SPI. Pm2 were over-expressed and tested against WSSV infection. By using hemocyte primary cell culture treated with each recombinant SPI. Pm2 domain along with WSSV, the expression of WSSV early genes ie1, WSV477 and late gene VP28 were substantially reduced as compared to other domains when the recombinant domain 2, rSPI. Pm2D2, was used. Injecting the WSSV along with rSPI. Pm2D2 but not with other domains caused delay in mortality rate of the infected shrimp. The results indicate that the SPI. Pm2D2 possesses strong antiviral activity and, hence, contributes predominantly to the antiviral activity of SPI. Pm2. © 2014 Elsevier Ltd.

Subject(s)

Primer DNA
 Serine proteinase inhibitor
 Amino acid sequence
 Animal
 Blood cell
 Cell culture
 Drug effects
 Evaluation study
 Gene expression regulation
 Genetics
 Immunology
 Innate immunity
 Metabolism
 Molecular genetics
 Penaeidae
 Polyacrylamide gel electrophoresis
 Protein tertiary structure
 Virology
 White spot syndrome virus
 Amino Acid Sequence
 Animals
 Cells, Cultured
 DNA Primers
 Electrophoresis, Polyacrylamide Gel
 Gene Expression Regulation, Viral
 Hemocytes
 Immunity, Innate
 Molecular Sequence Data
 Penaeidae
 Protein Structure, Tertiary
 Serine Proteinase Inhibitors
 White spot syndrome virus 1

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URI

https://hdl.handle.net/20.500.14740/6272

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Scopus: Year 1983-2021

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