Publication:
The presence of opioid receptors in rat inner ear

1
0

Issued Date

2003

Resource Type

Article

File Type

application/pdf

ISSN

3785955

DOI

10.1016/S0378-5955(03)00175-8

Other identifier(s)

2-s2.0-0037677544

Rights Holder(s)

Scopus

Bibliographic Citation

Hearing Research. Vol 181, (2003), p.85-93

Suggested Citation

Jongkamonwiwat N., Phansuwan-Pujito P., Sarapoke P., Chetsawang B., Casalotti S.O., Forge A., Dodson H., Govitrapong P. The presence of opioid receptors in rat inner ear. Hearing Research. Vol 181, (2003), p.85-93. doi:10.1016/S0378-5955(03)00175-8 Retrieved from: https://hdl.handle.net/20.500.14740/6667

Title

The presence of opioid receptors in rat inner ear

Author(s)

Jongkamonwiwat N.
 Phansuwan-Pujito P.
 Sarapoke P.
 Chetsawang B.
 Casalotti S.O.
 Forge A.
 Dodson H.
 Govitrapong P.

Abstract

Opioid peptides have been identified in the inner ear but relatively little information is available about the expression and distribution of their receptors. The aim of the present study was therefore to identify and localize the μ (MOR), δ (DOR) and κ (KOR) opioid receptor subtypes within the rat cochlea. The expression of these opioid receptor subtypes was determined by reverse transcriptase-polymerase chain reaction followed by nested polymerase chain reaction analysis. Amplification of RNAs from rat cerebral cortex (positive control) and rat cochlea with MOR, DOR and KOR primers resulted in products of the predicted lengths, 564, 356 and 276 bp, respectively. Restriction digestion confirmed the identity of these products. All three receptor subtypes were identified in the cochlea and further characterized by immunocytochemistry. DOR and KOR immunoreactivity was found in inner and outer hair cells, bipolar cells of the spiral ganglion and interdental cells of the limbus. In contrast, no MOR immunoreactivity was observed in the inner and outer hair cells, and interdental cells. All three types of receptor fibers were also detected in the bipolar cells and nerve fibers within the spiral ganglion. In addition, MOR- and KOR-containing nerve fibers were observed in the limbus. These findings are the first report of the presence of all three classical opioid receptors in the inner ear and suggest that these receptors may have both presynaptic and postsynaptic roles. © 2003 Elsevier Science B.V. All rights reserved.

Subject(s)

Delta opiate receptor
 Kappa opiate receptor
 Messenger RNA
 Mu opiate receptor
 Opiate receptor
 Receptor subtype
 Animal
 Animal experiment
 Article
 Brain cortex
 Cochlea
 Controlled study
 Cornea limbus
 Gene amplification
 Genetics
 Hair cell
 Immunocytochemistry
 Immunohistochemistry
 Immunoreactivity
 Inner ear
 Male
 Metabolism
 Nerve fiber
 Nonhuman
 Nucleotide sequence
 Polymerase chain reaction
 Priority journal
 Protein analysis
 Protein expression
 Protein localization
 Rat
 Rat strain
 Reverse transcription polymerase chain reaction
 Spiral ganglion
 Tissue distribution

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6667

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By