Publication:
Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene

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Issued Date

2002

Resource Type

Conference Paper

File Type

application/pdf

ISSN

9320113

DOI

10.1007/s00436-001-0567-2

Other identifier(s)

2-s2.0-0036318640

Rights Holder(s)

Scopus

Bibliographic Citation

Parasitology Research. Vol 88, No.SUPPL. 1 (2002), p.S33-S35

Suggested Citation

Chansiri K., Sarataphan N. Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene. Parasitology Research. Vol 88, No.SUPPL. 1 (2002), p.S33-S35. doi:10.1007/s00436-001-0567-2 Retrieved from: https://hdl.handle.net/20.500.14740/6747

Title

Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene

Author(s)

Chansiri K.
 Sarataphan N.

Abstract

Theileria type Thung Song is an indigenous hemoparasite of dairy cattle from the south of Thailand. It has previously been classified using the analysis of a comparative set of small subunit ribosomal RNA nucleotide sequences. However, the classification of this parasite is still questionable since the Theileria type Thung Song was located as the intermediate parasite between pathogenic and benign groups. We use the thymidylate synthetase gene (TS) as an alternative for the rapid molecular phylogenetic tree construction of benign Theileria type Thung Song, Theileria sergenti and Theileria buffeli with Babesia bovis as an out-group. The partial nucleotide sequences were determined using PCR, cloning and dideoxy sequencing. The TS nucleotide sequence data were aligned and analyzed by distance and maximum likelihood methods to construct the phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. All tree-building methods gave similar results. This study shows that T. sergenti and T. buffeli are closely related whereas Theileria type Thung Song is more distantly related.

Subject(s)

Protozoal protein
 Thymidylate synthase
 Protozoal DNA
 Thymidylate synthase
 Babesia bovis
 Conference paper
 Controlled study
 Genetic analysis
 Nonhuman
 Nucleotide sequence
 Phylogenetic tree construction method
 Priority journal
 Protozoal genetics
 Theileria
 Theileria buffeli
 Theileria sergenti
 Animal
 Animal parasitosis
 Article
 Cattle
 Cattle disease
 Classification
 DNA sequence
 Enzymology
 Genetics
 Molecular genetics
 Parasitology
 Pathogenicity
 Phylogeny
 Polymerase chain reaction
 Babesia
 Babesia bovis
 Bos taurus
 Theileria
 Theileria buffeli
 Theileria sergenti
 Theileria sp.
 Animals
 Cattle
 Cattle Diseases
 DNA, Protozoan
 Molecular Sequence Data
 Phylogeny
 Polymerase Chain Reaction
 Sequence Analysis, DNA
 Theileria
 Theileriasis
 Thymidylate Synthase

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URI

https://hdl.handle.net/20.500.14740/6747

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Scopus: Year 1983-2021

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