Publication:
P2X7 receptor-Pannexin1 interaction mediates stress-induced interleukin-1 beta expression in human periodontal ligament cells

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

223484

DOI

10.1111/jre.12139

Other identifier(s)

2-s2.0-84908596612

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Periodontal Research. Vol 49, No.5 (2014), p.595-602

Suggested Citation

Kanjanamekanant K., Luckprom P., Pavasant P. P2X7 receptor-Pannexin1 interaction mediates stress-induced interleukin-1 beta expression in human periodontal ligament cells. Journal of Periodontal Research. Vol 49, No.5 (2014), p.595-602. doi:10.1111/jre.12139 Retrieved from: https://hdl.handle.net/20.500.14740/6288

Title

P2X7 receptor-Pannexin1 interaction mediates stress-induced interleukin-1 beta expression in human periodontal ligament cells

Author(s)

Kanjanamekanant K.
 Luckprom P.
 Pavasant P.

Abstract

Background and Objective: Pannexin 1 (Panx1) has been found to form nonjunctional hemichannels. It is also proposed to combine with the P2X7 receptor, forming a complex involved in adenosine triphosphate (ATP)-induced interleukin-1beta (IL-1β) release in macrophages. Previously, we reported that mechanical stress induced IL-1β expression via the ATP/P2X7 receptor-dependent pathway in human periodontal ligament (HPDL) cells and that ATP was released through the connexin 43 (Cx43) hemichannel. In the present work, we examined the role of Panx1 in stress-induced IL-1β induction in HPDL cells. Material and Methods: Cultured HPDL cells were treated with compressive loading or ATP to stimulate IL-1β expression. Inhibitors, antagonists and the small interfering RNA technique were used to investigate the involvement of Panx1 in IL-1β induction. Co-immunoprecipitation (Co-IP) and immunostaining were used to determine the association of Panx1 with the P2X7 receptor. The IL-1β release mechanism was analyzed using inhibitors. Results: Blocking Panx1 significantly decreased ATP release, as well as IL-1β up-regulation, upon stimulation with stress or ATP. Co-IP revealed the association of Panx1 and the P2X7 receptor in HPDL cells, which was increased in response to mechanical loading. Pretreatment with vesicular trafficking inhibitors significantly reduced the amount of IL-1β released from stimulated cells, suggesting that IL-1β might be released through vesicles. Conclusion: We clearly illustrated the contribution of Panx1 in ATP release, as well as in IL-1β induction in HPDL cells. The association of Panx1 and the P2X7 receptor might be required for IL-1β induction, and their possible novel role in IL-1β vesicular release was indicated. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Subject(s)

Adenosine triphosphate
 Carbenoxolone
 Connexin 43
 Connexin 50
 Gap junction protein
 GJD4 protein, human
 Interleukin 1beta
 Meclofenamic acid
 Nerve protein
 PANX1 protein, human
 Probenecid
 Purinergic P2X7 receptor
 Quinine
 Small interfering RNA
 Spermine
 Antagonists and inhibitors
 Biomechanics
 Cell culture
 Culture technique
 Cytology
 Genetics
 Human
 Immunology
 Mechanical stress
 Periodontal ligament
 Adenosine Triphosphate
 Biomechanical Phenomena
 Carbenoxolone
 Cell Culture Techniques
 Cells, Cultured
 Connexin 43
 Connexins
 Humans
 Interleukin-1beta
 Meclofenamic Acid
 Nerve Tissue Proteins
 Periodontal Ligament
 Probenecid
 Quinine
 Receptors, Purinergic P2X7
 RNA, Small Interfering
 Spermine
 Stress, Mechanical

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URI

https://hdl.handle.net/20.500.14740/6288

Collections

Scopus: Year 1983-2021

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