Publication: A one-step rapid screening test of: Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
17599660
Other identifier(s)
2-s2.0-85035064952
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Analytical Methods. Vol 9, No.45 (2017), p.6403-6410
Suggested Citation
Wachiralurpan S., Sriyapai T., Areekit S., Sriyapai P., Thongphueak D., Santiwatanakul S., Chansiri K. A one-step rapid screening test of: Listeria monocytogenes in food samples using a real-time loop-mediated isothermal amplification turbidity assay. Analytical Methods. Vol 9, No.45 (2017), p.6403-6410. doi:10.1039/c7ay01750b Retrieved from: https://hdl.handle.net/20.500.14740/3976
Abstract
A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL-1 and 2.82 × 103 CFU mL-1, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products. © 2017 The Royal Society of Chemistry.