Publication:
Electrochemical detection of human papillomavirus DNA type 16 using a pyrrolidinyl peptide nucleic acid probe immobilized on screen-printed carbon electrodes

0
0

Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2013.11.023

Other identifier(s)

2-s2.0-84888784888

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Biosensors and Bioelectronics. Vol 54, (2014), p.428-434

Suggested Citation

Jampasa S., Wonsawat W., Rodthongkum N., Siangproh W., Yanatatsaneejit P., Vilaivan T., Chailapakul O. Electrochemical detection of human papillomavirus DNA type 16 using a pyrrolidinyl peptide nucleic acid probe immobilized on screen-printed carbon electrodes. Biosensors and Bioelectronics. Vol 54, (2014), p.428-434. doi:10.1016/j.bios.2013.11.023 Retrieved from: https://hdl.handle.net/20.500.14740/6840

Title

Electrochemical detection of human papillomavirus DNA type 16 using a pyrrolidinyl peptide nucleic acid probe immobilized on screen-printed carbon electrodes

Author(s)

Jampasa S.
 Wonsawat W.
 Rodthongkum N.
 Siangproh W.
 Yanatatsaneejit P.
 Vilaivan T.
 Chailapakul O.

Abstract

An electrochemical biosensor based on an immobilized anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe was successfully developed for the selective detection of human papillomavirus (HPV) type 16 DNA. A 14-mer acpcPNA capture probe was designed to recognize a specific 14 nucleotide region of HPV type 16 L1 gene. The redox-active label anthraquinone (AQ) was covalently attached to the N-terminus of the acpcPNA probe through an amide bond. The probe was immobilized onto a chitosan-modified disposable screen-printed carbon electrode via a C-terminal lysine residue using glutaraldehyde as a cross-linking agent. Hybridization with the target DNA was studied by measuring the electrochemical signal response of the AQ label using square-wave voltammetric analysis. The calibration curve exhibited a linear range between 0.02 and 12.0. μM with a limit of detection and limit of quantitation of 4 and 14. nM, respectively. This DNA sensing platform was successfully applied to detect the HPV type 16 DNA from a PCR amplified (240. bp fragment of the L1 gene) sample derived from the HPV type 16 positive human cancer cell line (SiHa), and failed to detect the HPV-negative c33a cell line. The sensor probe exhibited very high selectivity for the complementary 14 base oligonucleotide over the non-complementary oligonucleotides with sequences derived from HPV types 18, 31 and 33. The proposed sensor provides an inexpensive tool for the early stage detection of HPV type 16, which is an important biomarker for cervical cancer. © 2013 Elsevier B.V.

Subject(s)

Amino acids
 Aromatic compounds
 Cell culture
 Crosslinking
 DNA
 Drug products
 Electrodes
 Genes
 Ketones
 Oligonucleotides
 Peptides
 Probes
 Sensors
 AcpcPNA
 Anthraquinone
 ELectrochemical detection
 Human papillomavirus
 Screen printed electrodes
 Polymerase chain reaction
 Virus DNA
 Amino terminal sequence
 Article
 Binding affinity
 Carboxy terminal sequence
 Controlled study
 DNA cross linking
 DNA determination
 Electrochemistry
 Fluorescence microscopy
 High performance liquid chromatography
 Human papillomavirus type 16
 Nonhuman
 Nucleic acid probe
 Piezoelectricity
 Polymerase chain reaction
 Potentiometry
 Protein binding
 Protein conformation
 Sensitivity and specificity
 Human papillomavirus
 Human papillomavirus type 16
 AcpcPNA
 Anthraquinone
 Electrochemical detection
 Human papillomavirus
 Screen-printed electrode
 Anthraquinones
 Biosensing Techniques
 Carbon
 Cell Line, Tumor
 DNA, Viral
 Electrochemical Techniques
 Electrodes
 Equipment Design
 Human papillomavirus 16
 Humans
 Limit of Detection
 Nucleic Acid Hybridization
 Nucleic Acid Probes
 Papillomavirus Infections
 Peptide Nucleic Acids

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6840

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By