Publication:
Egg-Hatching Mechanism of Human Liver Fluke, Opisthorchis viverrini: A Role for Leucine Aminopeptidases from the Snail Host, Bithynia siamensis goniomphalos

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

223395

DOI

10.1645/16-125

Other identifier(s)

2-s2.0-85048268748

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Parasitology. Vol 104, No.4 (2018), p.388-397

Suggested Citation

Khampoosa P., Jones M.K., Lovas E.M., Piratae S., Kulsuntiwong J., Prasopdee S., Srisawangwong T., Laha T., Sripanidkulchai B., Thitapakorn V., Tesana S. Egg-Hatching Mechanism of Human Liver Fluke, Opisthorchis viverrini: A Role for Leucine Aminopeptidases from the Snail Host, Bithynia siamensis goniomphalos. Journal of Parasitology. Vol 104, No.4 (2018), p.388-397. doi:10.1645/16-125 Retrieved from: https://hdl.handle.net/20.500.14740/3681

Title

Egg-Hatching Mechanism of Human Liver Fluke, Opisthorchis viverrini: A Role for Leucine Aminopeptidases from the Snail Host, Bithynia siamensis goniomphalos

Author(s)

Khampoosa P.
 Jones M.K.
 Lovas E.M.
 Piratae S.
 Kulsuntiwong J.
 Prasopdee S.
 Srisawangwong T.
 Laha T.
 Sripanidkulchai B.
 Thitapakorn V.
 Tesana S.

Abstract

The human liver fluke Opisthorchis viverrini (Platyhelminthes, Trematoda, Digenea) uses snails of the genus Bithynia as first intermediate host. Peculiarly among trematodes, the eggs of O. viverrini hatch within the digestive tract of its snail host. It remains uncertain whether hatching in this species is mediated through mechanical fracture of the eggshell or by digestion with specific digestive enzymes. This study aimed to characterize enzymes with specific inhibitors and factors involved in the hatching activity of O. viverrini eggs. For measuring egg hatching in vivo, 50 O. viverrini mature eggs were fed to individual Bithynia siamensis goniomphalos snails at various temperature conditions for 24 hr. Ex vivo, mature eggs were incubated with crude snail extract and commercial leucine aminopeptidase (LAP). Egg-hatching of O. viverrini was temperature dependent, with optimal hatching occurring at 24-28 C, with a peak of hatching of 93.54% in vivo and 30.55% ex vivo occurring at these temperatures. Ex vivo hatching rates increased to 45.87% under anaerobic conditions at 28 C. Some 22.70% and 16.21% of heat-killed eggs also hatched within the snail digestive tract and snail extract, respectively, indicating that host molecules are involved in the hatching response. Most eggs hatch in the anterior regions of the digestive tract. Hatching was completely inhibited in the presence of bestatin, an inhibitor of LAP, but not in the presence of phosphatase inhibitors. Bestatin inhibition of hatching was reversible. Finally, egg hatching could be induced by addition of a porcine LAP. The results indicate that this digenean utilizes both LAP of the snail host and movement of miracidia for hatching. © American Society of Parasitologists 2018.

Subject(s)

Bestatin
 Cytosol aminopeptidase
 Cytosol aminopeptidase
 Enzyme inhibitor
 Leucine
 Protein phosphatase inhibitor-1
 Proteinase inhibitor
 Signal peptide
 Anoxic conditions
 Eggshell
 Enzyme
 Enzyme activity
 Host-parasite interaction
 Inhibition
 Inhibitor
 Intermediate disturbance hypothesis
 Invertebrate
 Parasite
 Snail
 Temperature effect
 Article
 Bithynia
 Bithynia siamensis goniomphalos
 Cercaria
 Controlled study
 Digestive system
 Egg shell
 Electron microscopy
 Enzymatic degradation
 Ex vivo study
 Exocrine gland
 Food contamination
 Hatching
 Miracidium
 Nonhuman
 Opisthorchis viverrini
 PH
 Surface property
 Analysis of variance
 Animal
 Drug effect
 Enzymology
 Female
 Human
 Male
 Metabolism
 Opisthorchis
 Ovum
 Parasitology
 Physiology
 Scanning electron microscopy
 Snail
 Transmission electron microscopy
 Ultrastructure
 Bithynia
 Digenea (flukes)
 Fasciola hepatica
 Gastropoda
 Opisthorchis viverrini
 Platyhelminthes
 Sus
 Trematoda
 Analysis of Variance
 Animals
 Cercaria
 Enzyme Inhibitors
 Female
 Humans
 Intracellular Signaling Peptides and Proteins
 Leucine
 Leucyl Aminopeptidase
 Male
 Microscopy, Electron, Scanning
 Microscopy, Electron, Transmission
 Opisthorchis
 Ovum
 Protease Inhibitors
 Snails

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https://hdl.handle.net/20.500.14740/3681

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Scopus: Year 1983-2021

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