Publication: Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
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Issued Date
2022
Resource Type
Language
eng
File Type
application/pdf
ISSN
1252526
Rights Holder(s)
Scopus
Bibliographic Citation
Infection and Drug Resistance. Vol 15, No. (2022), p.1305-1315
Suggested Citation
Chuavong W., Ponprateep S., Ajawatanawong P., Vatanavicharn T. Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge. Infection and Drug Resistance. Vol 15, No. (2022), p.1305-1315. doi:10.12982/CMJS.2022.109 Retrieved from: https://hdl.handle.net/20.500.14740/9921
Abstract
Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confirmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rRNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modified M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, efficient PHB producers, exhibited the potential for their utilization in commercial PHB production. © 2022, Chiang Mai University. All rights reserved.