Publication:
Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

1355557X

DOI

10.1111/are.13894

Other identifier(s)

2-s2.0-85056738592

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Aquaculture Research. Vol 50, No.1 (2019), p.277-283

Suggested Citation

Soonthonsrima T., Wangman P., Chaivisuthangkura P., Pengsuk C., Sithigorngul P., Longyant S. Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus. Aquaculture Research. Vol 50, No.1 (2019), p.277-283. doi:10.1111/are.13894 Retrieved from: https://hdl.handle.net/20.500.14740/5558

Title

Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus

Author(s)

Soonthonsrima T.
 Wangman P.
 Chaivisuthangkura P.
 Pengsuk C.
 Sithigorngul P.
 Longyant S.

Abstract

The simple immunoprecipitation method was used to isolate tilapia immunoglobulin (Ig) for immunization in order to produce monoclonal antibodies (MAbs) specific to tilapia Ig. First, the tilapia antiserum against bovine serum albumin (BSA) was prepared by peritoneal injection of BSA into tilapia, and the tilapia anti-BSA antiserum was used to precipitate BSA to form the Ig/BSA immune complex. The Ig/BSA immune complex was then injected into Swiss mice for hybridoma production. After fusion, three hybridoma clones producing MAbs specific to the tilapia antibody were selected by dot blot and Western blot. All MAbs (101A, 59G, and 11A) were bound specifically to the heavy chain of immunoglobulin M (IgM). The MAbs 101A and 59G demonstrated twofold higher affinity than MAb 11A and the commercialized antibody. However, MAbs 11A could also bind to the heavy chain of IgM in Asian seabass, Lates calcarifer, as well. These MAbs can be used to monitor the immune responses of individual fish by indirect ELISA upon exposure to various antigens. © 2018 John Wiley & Sons Ltd

Subject(s)

Antibody
 Antigen
 Cichlid
 Detection method
 Immune response
 Immunoassay
 Protein
 Bovinae
 Lates calcarifer
 Mus
 Oreochromis niloticus
 Tilapia

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URI

https://hdl.handle.net/20.500.14740/5558

Collections

Scopus: Year 1983-2021

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