Publication: Colorimetric and fluorescence detection of Pb<sup>2+</sup> by fluorescein derivative
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Issued Date
2025-02-15
Resource Type
ISSN
13861425
Scopus ID
2-s2.0-85208289452
Pubmed ID
39522228
Journal Title
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume
327
Rights Holder(s)
SCOPUS
Bibliographic Citation
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Vol.327 (2025)
Suggested Citation
Chaivisuthangkura T., Boosamund N., Asawutmangkul N., Khammee T., Jityuti B., Siangproh W. Colorimetric and fluorescence detection of Pb<sup>2+</sup> by fluorescein derivative. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Vol.327 (2025). doi:10.1016/j.saa.2024.125373 Retrieved from: https://hdl.handle.net/20.500.14740/20607
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Abstract
A simple and low cost method for rapid detection of Pb2+ was developed in this study using a simple fluorescent probe, biotin-4-fluorescein. The presence of Pb2+ induced the color change of fluorescein from yellow to pink which can be detected visually by naked eyes. The color change of biotin-4-fluorescein was not observed while testing with other metal ions, such as Fe3+, Cu2+, Ca2+, Co2+ and Cd2+, indicating the selectivity of fluorescein derivative with Pb2+. Moreover, the color change was observed at pH above 7.0, and the pink color got more intense when increasing pH. Adding EDTA, a chelating reagent, into the solution of biotin-4-fluorescein/Pb2+ resulted to the rebound of the color to the original color of free biotin-4-fluorescein. The fluorescence spectra of fluorescein decreased with increasing Pb2+concentration, and the quenching was enhanced at higher pH. The association constant (Ka) of 2.00 × 104 M−1 was calculated from the fluorescence spectra with the limit of detection (LOD) and the limit of quantitation (LOQ) of 1.38 × 10−5 M (2.86 ppm) and 4.61 × 10−5 M (9.55 ppm), respectively. Job's plot analysis indicated 2:1 ratio of binding interaction. Pb2+ in untreated wastewater was quantified for 24.99 ppm using this method. This quick analysis can be beneficial for the application of lead detection in real sample solutions, such as wastewater from industrial factories.