Publication:
Differential expression of CMG peptide and crustacean hyperglycemic hormones (CHHs) in the eyestalk of the giant tiger prawn Penaeus monodon

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

1969781

DOI

10.1016/S0196-9781(02)00180-8

Other identifier(s)

2-s2.0-0036848248

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Peptides. Vol 23, No.11 (2002), p.1943-1952

Suggested Citation

Sithigorngul P., Panchan N., Chaivisuthangkura P., Longyant S., Sithigorngul W., Petsom A. Differential expression of CMG peptide and crustacean hyperglycemic hormones (CHHs) in the eyestalk of the giant tiger prawn Penaeus monodon. Peptides. Vol 23, No.11 (2002), p.1943-1952. doi:10.1016/S0196-9781(02)00180-8 Retrieved from: https://hdl.handle.net/20.500.14740/6688

Title

Differential expression of CMG peptide and crustacean hyperglycemic hormones (CHHs) in the eyestalk of the giant tiger prawn Penaeus monodon

Author(s)

Sithigorngul P.
 Panchan N.
 Chaivisuthangkura P.
 Longyant S.
 Sithigorngul W.
 Petsom A.

Abstract

Mouse antiserum against C-terminal amide of Pem-CMG (a peptide in the family of CHH/MIH/GIH) penta-deca peptide (RPRQRNQYRAALQRLamide=CMG-15) was generated and used for localization of the peptide in tissue and extract of the eyestalk of Penaeus monodon by means of immunohistochemistry and dot-ELISA in comparison with anti-T+ antiserum (T+=YANAVQTVamide: the putative C-terminal amide of crustacean hyperglycemic hormone (CHH) of Macrobrachium rosenbergii). The anti-CMG-15 antiserum did not show cross-reactivity to T+ peptide by dot-ELISA and vice versa for anti-T+ antiserum. In dot-ELISA of eyestalk extract of P. monodon after one step separation by RP-HPLC, anti-CMG-15 antiserum recognized different peptide fractions (F38-39) from those recognized by anti-T+ antiserum (F19, 40-41 and 47-51). Most of the T+ immunoreactive fractions (except F19) show higher hyperglycemic activity than the CMG immunoreactive fractions. In immunohistochemical localization, anti-CMG antiserum recognized only 2-3 neurons in medulla terminalis X-organ complex (MTXO) with long processes terminated in the sinus gland. The CMG-immunoreactive neurons were clearly distinct from CHH containing neurons situated in the same area. This evidence confirms the existing of CMG peptide which may play distinct roles from CHHs in hormonal regulation in P. monodon. © 2002 Elsevier Science Inc. All rights reserved.

Subject(s)

Antiserum
 Arginylpropylarginylglutaminylarginylasparaginylglutaminyltyrosylarginylala nylalanylleucylglutaminylarginylleucinamide
 Peptide antibody
 Polypeptide
 Tyrosylalanylasparaginylalanylvalylglutaminylthreonylvalinamide
 Unclassified drug
 Hyperglycemic hormone, crustacean
 Nerve protein
 Article
 Controlled study
 Cross reaction
 Crustacea
 Enzyme linked immunosorbent assay
 Eye
 Hormonal regulation
 Immunocompetent cell
 Immunohistochemistry
 Immunoreactivity
 Nerve cell
 Nonhuman
 Priority journal
 Protein expression
 Protein localization
 Tiger
 Tissue distribution
 Amino acid sequence
 Animal
 Chemistry
 Eye
 Immunology
 Metabolism
 Molecular genetics
 Sequence homology
 Crustacea
 Macrobrachium
 Macrobrachium rosenbergii
 Monodon
 Panthera tigris
 Penaeus monodon
 Amino Acid Sequence
 Animals
 Crustacea
 Enzyme-Linked Immunosorbent Assay
 Eye
 Immune Sera
 Immunohistochemistry
 Molecular Sequence Data
 Nerve Tissue Proteins
 Sequence Homology, Amino Acid

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URI

https://hdl.handle.net/20.500.14740/6688

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Scopus: Year 1983-2021

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