Publication:
Photocleavage of avidin by a new pyrenyl probe

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

10111344

DOI

10.1016/j.jphotobiol.2011.04.003

Other identifier(s)

2-s2.0-79955937531

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Photochemistry and Photobiology B: Biology. Vol 103, No.3 (2011), p.251-255

Suggested Citation

Malaikaew P., Svasti J., Kumar C.V., Buranaprapuk A. Photocleavage of avidin by a new pyrenyl probe. Journal of Photochemistry and Photobiology B: Biology. Vol 103, No.3 (2011), p.251-255. doi:10.1016/j.jphotobiol.2011.04.003 Retrieved from: https://hdl.handle.net/20.500.14740/7316

Title

Photocleavage of avidin by a new pyrenyl probe

Author(s)

Malaikaew P.
 Svasti J.
 Kumar C.V.
 Buranaprapuk A.

Abstract

In this study, a new small-molecule-based reagent was designed to recognize and bind to specific site in protein. A new pyrenyl probe, d-biotinyl-1(1- pyrene)methylamide (Py-biotin) was designed and synthesized by coupling of d-biotin to 1(1-pyrene)methylamine hydrochloride. Binding studies and site-specific photocleavage of avidin by Py-biotin were demonstrated. Binding of Py-biotin to avidin was studied using absorbance and fluorescence spectroscopic techniques. Red shifts of the absorption peak positions of the pyrenyl chromophore followed by hyperchromism were observed upon binding to avidin. The photocleavage of avidin was achieved when a mixture of the protein, Py-biotin, and an electron acceptor, cobalt(III) hexammine trichloride (CoHA), was irradiated at 342 nm. No reaction occurred in the absence of the probe, CoHA, or light. N-terminal sequencing of the peptide fragments indicated a cleavage site of avidin between Thr 77 and Val 78. The high specificity of photocleavage may be valuable in targeting specific sites of proteins with small molecules. © 2011 Elsevier B.V. All rights reserved.

Subject(s)

Avidin
 Biotin
 Peptide fragment
 Amino terminal sequence
 Article
 Binding site
 Chromatophore
 Fluorescence spectroscopy
 Irradiation
 Molecular library
 Photochemistry
 Photodynamics
 Priority journal
 Protein binding
 Protein structure
 Protein targeting
 Absorption
 Avidin
 Binding Sites
 Biotin
 Chlorides
 Cobalt
 Electrophoresis, Polyacrylamide Gel
 Fluorescent Dyes
 Light
 Peptide Fragments
 Photolysis
 Pyrenes
 Spectrometry, Fluorescence
 Threonine
 Valine

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URI

https://hdl.handle.net/20.500.14740/7316

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Scopus: Year 1983-2021

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