Publication: Two-generation exposure to a high-fat diet induces the change of salty taste preference in rats
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Issued Date
2023
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application/pdf
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Scopus
Bibliographic Citation
Scientific Reports. Vol 13, No.1 (2023)
Suggested Citation
Serirukchutarungsee S., Watari I., Narukawa M., Podyma-Inoue K.A., Sangsuriyothai P., Ono T. Two-generation exposure to a high-fat diet induces the change of salty taste preference in rats. Scientific Reports. Vol 13, No.1 (2023). doi:10.1038/s41598-023-31662-0 Retrieved from: https://hdl.handle.net/20.500.14740/10519
Other Contributor(s)
Abstract
High-fat diet (HFD) leads to multiple complications, including taste alteration. This study observed the effect of a two-generation exposure to an HFD on the peripheral taste system in offspring. Ten pregnant Wistar rats were assigned a standard diet (SD) (n = 5) or HFD (n = 5) from day 7 of pregnancy through the lactation. Thirty-six male and female 3-week-old offspring were measured for body weight and blood glucose level, and the circumvallate papillae were collected. The other twenty-four 3-week-old offspring were weaned on the same diet as their mothers and raised individually. The taste preference behaviors were studied using the two-bottle taste preference test and analyzed five basic tastes (sweet, bitter, umami, sour, and salty). The expressions of epithelial sodium channel alpha subunit (ENaCα) and angiotensin II receptor type 1 (AT1) in the circumvallate papilla were analyzed by immunohistochemical staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We found increased body weight and salty taste preference of offspring from the HFD group in both sexes. Correspondingly, the AT1 level of the taste bud cells significantly increased in 3-week-old female offspring from the HFD group. An increase in AT1 levels may be a risk factor for changes in salty taste preference. © 2023, The Author(s).