Publication: Development of a monoclonal antibody specific to yellow head virus (YHV) from Penaeus monodon
2
0
Issued Date
2000
Resource Type
File Type
application/pdf
ISSN
1775103
Other identifier(s)
2-s2.0-0034632697
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Diseases of Aquatic Organisms. Vol 42, No.1 (2000), p.27-34
Suggested Citation
Sithigorngul P., Chauychuwong P., Sithigorngul W., Longyant S., Chaivisuthangkura P., Menasveta P. Development of a monoclonal antibody specific to yellow head virus (YHV) from Penaeus monodon. Diseases of Aquatic Organisms. Vol 42, No.1 (2000), p.27-34. doi:10.3354/dao042027 Retrieved from: https://hdl.handle.net/20.500.14740/7009
Abstract
A monoclonal antibody specific to yellow head virus (YHV) was produced from a mouse immunized with gill extracts prepared from laboratory-reared Penaeus monodon dually infected with YHV and white spot syndrome virus (WSSV). One clone designated V3-2B specifically bound to native and SDS-treated viral specific antigens. Immunocytochemical studies of infected gills revealed viral specific immunoreactivities in the cytoplasm of gill tissue and in haemocytes. No antibody binding was observed in gills from non-infected shrimp. In addition, immunocytochemical examination of tissues from shrimp experimentally infected with YHV gave a positive reaction, while tissues from uninfected control shrimp or shrimp experimentally infected with WSSV did not. Western blot analysis indicated that the antibody reacted with a protein of approximately 135 kD that was present only in shrimp infected with YHV. In dot-blot indirect immunoperoxidase assays, the antibody was able to detect viral associated antigen in diluted haemolymph up to 1:50 dilution and in an ammonium sulfate precipitate of haemolymph up to 1:1000 dilution. The results suggested that this antibody might be useful for development of effective diagnostic techniques for both heavy and mild YHV infections in shrimp.
Subject(s)
Ammonium sulfate
Antibody specificity
Antigen detection
Clone
Dilution
Dodecyl sulfate sodium
Immunodiagnosis
Immunoperoxidase technique
Monoclonal antibody
Mouse
White spot syndrome virus
Yellow head virus
Decapoda (Crustacea)
Monodon
Penaeus monodon
Penaeus monodon
Shrimp white spot syndrome virus
Yellow head virus
Antibody specificity
Antigen detection
Clone
Dilution
Dodecyl sulfate sodium
Immunodiagnosis
Immunoperoxidase technique
Monoclonal antibody
Mouse
White spot syndrome virus
Yellow head virus
Decapoda (Crustacea)
Monodon
Penaeus monodon
Penaeus monodon
Shrimp white spot syndrome virus
Yellow head virus