Publication:
Alpha-mangostin partially preserves expression of ammonia-metabolizing enzymes in thioacetamide- induced fibrotic and cirrhotic rats

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Issued Date

2015

Resource Type

Article

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application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84957676182

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Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 98, (2015), p.S53-S60

Suggested Citation

Khunvirojpanich M., Showpittapornchai U., Moongkarndi P., Pradidarcheep W. Alpha-mangostin partially preserves expression of ammonia-metabolizing enzymes in thioacetamide- induced fibrotic and cirrhotic rats. Journal of the Medical Association of Thailand. Vol 98, (2015), p.S53-S60. Retrieved from: https://hdl.handle.net/20.500.14740/6069

Title

Alpha-mangostin partially preserves expression of ammonia-metabolizing enzymes in thioacetamide- induced fibrotic and cirrhotic rats

Author(s)

Khunvirojpanich M.
 Showpittapornchai U.
 Moongkarndi P.
 Pradidarcheep W.

Abstract

Background: Ammonia metabolizing enzymes, carbamyol phosphate synthetase (CPS) and glutamine synthetase (GS), are expressed in the periportal and pericentral hepatocytes, respectively. CPS and GS function complementary to ensure complete ammonia detoxification. Immunohistochemical analysis confirmed the decline of both CPS and GS in cirrhotic rat liver induced by thioacetamide (TAA). Alpha-mangostin (AM), a major derivative of xanthone from mangosteen, has been reported to possess a wide range of pharmacological properties. Objective: To examine the preventive effects of AM on CPS and GS expression in fibrotic and cirrhotic rats induced by TAA over sixteen weeks. Material and Method: Twenty-four male Wistar rats were divided into 4 groups of 6 animals each. Group 1 was for control. Group 2 was for pure TAA treatment. Group 3 was for pure AM administration. Group 4, prevention group, was concurrently treated with TAA and AM. Immunohistochemical technique was employed in order to elucidate the expression of CPS and GS in each animal group. Results: Immunohistochemical staining for CPS and GS showed an increasing decline from week eight to sixteen under pure- TAA condition. Fibrous bridgings, nodule formations, and regenerative nodules were detected. Pure-AM condition yielded strongly CPS and GS-stained hepatocytes in a fashion similar to the control. Results from the prevention group showed a decreasing decline of CPS and GS immuno-reactivity from week eight to sixteen as compared to pure-TAA condition. Fewer fibrous portal-caval bridgings were observed at week eight and CPS-positive hepatocytes were found in continuous rings. Conclusion: Alpha-mangostin could partially preserve the normal expression of ammonia-metabolizing enzymes under TAA-induced fibrotic and cirrhotic conditions. © 2015, Medical Association of Thailand. All rights reserved.

Subject(s)

Alpha mangostin
 Carbamoyl phosphate synthase
 Glutamate ammonia ligase
 Thioacetamide
 Unclassified drug
 Xanthone derivative
 Ammonia
 Glutamate ammonia ligase
 Mangostin
 Xanthone derivative
 Animal experiment
 Animal model
 Animal tissue
 Article
 Central vein
 Controlled study
 Histology
 Immunohistochemistry
 Immunoreactivity
 Liver cell
 Liver cirrhosis
 Liver nodule
 Liver protection
 Male
 Neuroectoderm
 Nonhuman
 Perineural vascular plexus
 Prevention and control
 Protein expression
 Rat
 Thioacetamide-induced liver fibrosis
 Tissue fixation
 Animal
 Liver cirrhosis
 Metabolism
 Wistar rat
 Ammonia
 Animals
 Glutamate-Ammonia Ligase
 Hepatocytes
 Liver Cirrhosis
 Male
 Rats
 Rats, Wistar
 Thioacetamide
 Xanthones

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https://hdl.handle.net/20.500.14740/6069

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