Publication: Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
14625814
Other identifier(s)
2-s2.0-85090950583
Rights
Srinakharinwirot University
Rights Holder(s)
Scopus
Bibliographic Citation
Cellular Microbiology. Vol 22, No.12 (2020)
Suggested Citation
Somrit M., Yu S.-Y., Le Pendu J., Breiman A., Guérardel Y., Weerachatyanukul W., Watthammawut A. Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn. Cellular Microbiology. Vol 22, No.12 (2020). doi:10.1111/cmi.13258 Retrieved from: https://hdl.handle.net/20.500.14740/5371
Abstract
The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles. However, little is known about the host surface molecules to which MrNV attach to initiate infection. Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills. We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F. The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins. Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans. Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections. © 2020 John Wiley & Sons Ltd
Subject(s)
Fucose
Glycan
Glycoprotein
Lectin
Periodate
Aleuria aurantia
Animal cell
Animal tissue
Article
Binding site
Cell lysate
Controlled study
Freshwater species
Fucosylation
Gill
Macrobrachium rosenbergii
Mass spectrometry
Nodaviridae
Nonhuman
Priority journal
Protein interaction
Tissue section
Virus attachment
Virus like agent
Glycan
Glycoprotein
Lectin
Periodate
Aleuria aurantia
Animal cell
Animal tissue
Article
Binding site
Cell lysate
Controlled study
Freshwater species
Fucosylation
Gill
Macrobrachium rosenbergii
Mass spectrometry
Nodaviridae
Nonhuman
Priority journal
Protein interaction
Tissue section
Virus attachment
Virus like agent