Publication:
Sensory acceptable equivalent doses of β-phenylethyl isothiocyanate (PEITC) induce cell cycle arrest and retard the growth of p53 mutated oral cancer in vitro and in vivo

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

20426496

DOI

10.1039/c8fo00865e

Other identifier(s)

2-s2.0-85050534112

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Food and Function. Vol 9, No.7 (2018), p.3640-3656

Suggested Citation

Lam-Ubol A., Fitzgerald A.L., Ritdej A., Phonyiam T., Zhang H., Myers J.N., Huang P., Trachootham D. Sensory acceptable equivalent doses of β-phenylethyl isothiocyanate (PEITC) induce cell cycle arrest and retard the growth of p53 mutated oral cancer in vitro and in vivo. Food and Function. Vol 9, No.7 (2018), p.3640-3656. doi:10.1039/c8fo00865e Retrieved from: https://hdl.handle.net/20.500.14740/4101

Title

Sensory acceptable equivalent doses of β-phenylethyl isothiocyanate (PEITC) induce cell cycle arrest and retard the growth of p53 mutated oral cancer in vitro and in vivo

Author(s)

Lam-Ubol A.
 Fitzgerald A.L.
 Ritdej A.
 Phonyiam T.
 Zhang H.
 Myers J.N.
 Huang P.
 Trachootham D.

Abstract

High doses of β-phenylethyl isothiocyanate (PEITC), a phytochemical in cruciferous vegetables, are not feasible for consumption due to a strong mouth-tingling effect. This study investigated the anti-cancer effect of PEITC at sensory acceptable doses. In vitro, PEITC was selectively toxic to oral cancer cells (CAL-27, FaDu, SCC4, SCC 9, SCC15, SCC25 and TU138), compared to oral keratinocytes (OKF6/TERT2 and NOK/Si). In vivo, 5 and 10 mg kg-1 PEITC, equivalent to human organoleptically acceptable doses, retarded tumor growth and prolonged the survival of mice bearing p53-mutated oral cancer cells-TU138 xenograft. Mechanistically, PEITC induced ROS accumulation, nuclear translocation of p53 and p21 and G1/S cell cycle arrest in vitro; increased p53 and 8-oxo-dG levels; and decreased Ki-67 intense/mild staining ratios without TUNEL changes in vivo. These findings suggested that the sensory acceptable doses of PEITC selectively induced ROS-mediated cell cycle arrest leading to delayed tumor progression and extended survival. PEITC could be a functional ingredient for oral cancer prevention. © The Royal Society of Chemistry.

Subject(s)

Cytology
 Diseases
 Tumors
 Cell-cycle arrest
 Cruciferous vegetables
 Equivalent dose
 Functional ingredient
 Isothiocyanates
 Nuclear translocations
 Oral cancer cells
 Tumor progressions
 Cells
 Antineoplastic agent
 Isothiocyanic acid derivative
 Phenethyl isothiocyanate
 Protein p53
 Animal
 Apoptosis
 Cell cycle checkpoint
 Cell proliferation
 Drug effect
 Genetics
 Human
 Male
 Metabolism
 Mouse
 Mouth tumor
 Nude mouse
 Pathophysiology
 Taste
 Tumor cell line
 Animals
 Anticarcinogenic Agents
 Apoptosis
 Cell Cycle Checkpoints
 Cell Line, Tumor
 Cell Proliferation
 Humans
 Isothiocyanates
 Male
 Mice
 Mice, Nude
 Mouth Neoplasms
 Taste
 Tumor Suppressor Protein p53

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URI

https://hdl.handle.net/20.500.14740/4101

Collections

Scopus: Year 1983-2021

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