Publication:
Inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven Bhutanese medicinal plants

0
0

Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

3788741

DOI

10.1016/j.jep.2013.05.055

Other identifier(s)

2-s2.0-84880314176

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Ethnopharmacology. Vol 148, No.3 (2013), p.1013-1017

Suggested Citation

Wangchuk P., Keller P.A., Pyne S.G., Taweechotipatr M. Inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven Bhutanese medicinal plants. Journal of Ethnopharmacology. Vol 148, No.3 (2013), p.1013-1017. doi:10.1016/j.jep.2013.05.055 Retrieved from: https://hdl.handle.net/20.500.14740/6609

Title

Inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven Bhutanese medicinal plants

Author(s)

Wangchuk P.
 Keller P.A.
 Pyne S.G.
 Taweechotipatr M.

Abstract

Ethnopharmacological relevance Seven studied medicinal plants; Aconitum laciniatum, Ajania nubigena, Codonopsis bhutanica, Corydalis crispa, Corydalis dubia, Meconopsis simplicifolia and Pleurospermum amabile, are currently used in the Bhutanese Traditional Medicine (BTM) for the management of different types of disorders including the diseases that bore relevance to various inflammatory conditions. Aims of the study This study aimed to evaluate the inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven selected Bhutanese medicinal plants. It is expected to; (a) generate a scientific basis for their use in the BTM and (b) form a basis for prioritization of the seven plants for further phytochemical and anti-inflammatory studies. Materials and methods Seven plants were selected using an ethno-directed bio-rational approach and their crude extracts were prepared using four different solvents (methanol, hexane, dichloromethane and chloroform). The TNF-α inhibitory activity of these extracts was determined by cytokine-specific sandwich quantitative enzyme-linked immunosorbent assays (ELISAs). The results were quantified statistically and the statistical significance were evaluated by GraphPad Prism version 5.01 using Student's t-test with one-tailed distribution. A p-value ≤0.05 was considered statistically significant. Results Of the seven plants studied, the crude extracts of six of them inhibited the production of pro-inflammatory cytokine, TNF-α in LPS-activated THP-1 monocytic cells. Amongst the six plants, Corydalis crispa gave the best inhibitory activity followed by Pleurospermum amabile, Ajania nubigena, Corydalis dubia, Meconopsis simplicifolia and Codonopsis bhutanica. Of the 13 extracts that exhibited statistically significant TNF-α inhibitory activity (p<0.05; p<0.01), five of them showed very strong inhibition when compared to the DMSO control and RPMI media. Conclusions Six medicinal plants studied here showed promising TNF-α inhibitory activity. These findings rationalize the traditional use of these selected medicinal plants in the BTM as an individual plant or in combination with other ingredients for the treatment of disorders bearing relevance to the inflammatory conditions. The results forms a good preliminary basis for the prioritization of candidate plant species for an in-depth phytochemical study and anti-inflammatory activity screening of the pure compounds contained within those seven plants. © 2013 Elsevier Ireland Ltd.

Subject(s)

Aconitum laciniatum extract
 Ajania nubigena extract
 Antiinflammatory agent
 Codonopsis bhutanica extract
 Corydalis crispa extract
 Corydalis dubia extract
 Lipopolysaccharide
 Meconopsis simplicifolia extract
 Plant extract
 Pleurospermum amabile extract
 Tumor necrosis factor alpha
 Unclassified drug
 Aconitum laciniatum
 Ajania nubigena
 Antiinflammatory activity
 Article
 Codonopsis bhutanica
 Controlled study
 Corydalis crispa
 Corydalis dubia
 Cytokine production
 Double antibody sandwich enzyme linked immunosorbent assay
 Drug inhibition
 Human
 Human cell
 Meconopsis simplicifolia
 Medicinal plant
 Monocyte macrophage precursor cell
 Pleurospermum amabile
 Student t test
 Anti-inflammatory
 Bhutanese traditional medicine
 Medicinal plants
 TNF-α inhibition
 Angiosperms
 Anti-Inflammatory Agents
 Bhutan
 Cell Line
 Humans
 Lipopolysaccharides
 Medicine, Traditional
 Monocytes
 Plant Extracts
 Tumor Necrosis Factor-alpha
 Aconitum
 Ajania
 Codonopsis
 Corydalis
 Meconopsis simplicifolia
 Pleurospermum

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6609

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By