Publication: Extramedullary hematopoiesis in rat spleen after exposure to high doses of alpha-mangostin
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85075013073
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S185-S194
Suggested Citation
Pongsawat S., Jaruchotiratanasakul N., Nilbu-Nga C., Pradidarcheep W. Extramedullary hematopoiesis in rat spleen after exposure to high doses of alpha-mangostin. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S185-S194. Retrieved from: https://hdl.handle.net/20.500.14740/4301
Abstract
Background: The pericarp extract of mangosteen (Garicinia mangostana) Linn, alpha-mangostin, is known to have beneficial effects on the body which includes anti-inflammatory, as well as anti-diabetic properties. Extramedullary hematopoiesis is the process whereby blood cellular components are formed outside the bone marrow under stressful conditions. Objective: To evaluate the effects of subacute toxicity induced by alpha-mangostin in rat spleen. Material and Method: A total of 40 Wistar rats were used to study histopathological changes in the spleen after being exposed to alpha-mangostin for one month. The rats were equally divided into four groups: treated male (n = 10); treated female (n = 10); control male (n = 10); and control female (n = 10). The treated group received alpha-mangostin pericarp extract diluted in 0.1% carboxymethylcellulose (0.1% CMC) in a concentration of 100 mg/5 ml, and 100 mg/kg/day alpha-mangostin was introduced to the rats via intra-peritoneal injection. The control group received 0.1% CMC five days per week for one month as standard treatment. Splenic tissues were collected at the end of the study period. Paraffin sections were examined by H&E and immunohistochemical stainings. Body weight was measured before and after the intervention for all groups. Results: After exposure to alpha-mangostin, the treated groups showed increased platelet formation, myeloid progenitor cells, and megakaryocyte in the spleen as well as decrease in the body weight of male rats when compared to the control groups. Immunohistochemical study confirmed that the newly formed blood cells were from myeloid and erythroid precursor cells. Conclusion: Exposure to high dose of alpha-mangostin marked the presence of extramedullary hematopoiesis which is the formation of blood cellular components outside the bone marrow. © 2017 Medical Association of Thailand. All rights reserved.
Subject(s)
Alpha mangostin
Phytochemical
Unclassified drug
Animal experiment
Animal model
Animal tissue
Antidiabetic activity
Antiinflammatory activity
Article
Body weight
Bone marrow
Controlled study
Drug megadose
Extramedullary hematopoiesis
Female
Garcinia mangostana
High performance liquid chromatography
Histopathology
Immunohistochemistry
Male
Megakaryocyte
Myeloid progenitor cell
Nonhuman
Rat
Spleen
Thrombocytopoiesis
Toxicity
Phytochemical
Unclassified drug
Animal experiment
Animal model
Animal tissue
Antidiabetic activity
Antiinflammatory activity
Article
Body weight
Bone marrow
Controlled study
Drug megadose
Extramedullary hematopoiesis
Female
Garcinia mangostana
High performance liquid chromatography
Histopathology
Immunohistochemistry
Male
Megakaryocyte
Myeloid progenitor cell
Nonhuman
Rat
Spleen
Thrombocytopoiesis
Toxicity