Publication:
High-risk human papillomavirus genotype detection by electrochemical dna chip method

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

15137368

DOI

10.7314/APJCP.2012.13.4.1151

Other identifier(s)

2-s2.0-84873049778

Rights Holder(s)

Scopus

Bibliographic Citation

Asian Pacific Journal of Cancer Prevention. Vol 13, No.4 (2012), p.1151-1158

Suggested Citation

Chansaenroj J., Theamboonlers A., Chinchai T., Pairoj J., Swangvaree S., Karalak A., Takahashi M., Nikaido M., Gemma N., Poovorawan Y. High-risk human papillomavirus genotype detection by electrochemical dna chip method. Asian Pacific Journal of Cancer Prevention. Vol 13, No.4 (2012), p.1151-1158. doi:10.7314/APJCP.2012.13.4.1151 Retrieved from: https://hdl.handle.net/20.500.14740/7166

Title

High-risk human papillomavirus genotype detection by electrochemical dna chip method

Author(s)

Chansaenroj J.
 Theamboonlers A.
 Chinchai T.
 Pairoj J.
 Swangvaree S.
 Karalak A.
 Takahashi M.
 Nikaido M.
 Gemma N.
 Poovorawan Y.

Abstract

High-risk human papillomavirus (HPV) genotypes are the major cause of cervical cancer. Hence, HPV genotype detection is a helpful preventive measure to combat cervical cancer. Recently, several HPV detection methods have been developed, each with different sensitivities and specificities. The objective of this study was to compare HPV high risk genotype detection by an electrochemical DNA chip system, a line probe assay (INNOLiPA) and sequencing of the L1, E1 regions. A total of 361 cervical smears with different cytological findings were subjected to polymerase chain reaction-sequencing and electrochemical DNA chip assessment. Multiple infections were found in 21.9% (79/361) of the specimens, most prevalently in 20-29-year olds while the highest prevalence of HPV infection was found in the 30-39-year age group. The most prevalent genotype was HPV 16 at 28.2% (138/489) followed by HPV 52 at 9.6% (47/489), with the other types occurring at less than 9.0%. The electrochemical DNA chip results were compared with INNO-LiPA and sequencing (E1 and L1 regions) based on random selection of 273 specimens. The results obtained by the three methods were in agreement except for three cases. Direct sequencing detected only one predominant genotype including low risk HPV genotypes. INNO-LiPA identified multiple infections with various specific genotypes including some unclassified-risk genotypes. The electrochemical DNA chip was highly accurate, suitable for detection of single and multiple infections, allowed rapid detection, was less time-consuming and was easier to perform when compared with the other methods. It is concluded that for clinical and epidemiological studies, all genotyping methods are perfectly suitable and provide comparable results.

Subject(s)

Virus DNA
 Adult
 Aged
 Article
 Carcinoma in situ
 Comparative study
 DNA microarray
 DNA probe
 Electrochemical analysis
 Female
 Genetics
 Genotype
 Genotyping technique
 Human
 Isolation and purification
 Middle aged
 Papilloma virus
 Papillomavirus infection
 Pathology
 Polymerase chain reaction
 Uterine cervix
 Uterine cervix tumor
 Virology
 Adult
 Aged
 Aged, 80 and over
 Carcinoma in Situ
 Cervix Uteri
 DNA Probes, HPV
 DNA, Viral
 Electrochemical Techniques
 Female
 Genotype
 Genotyping Techniques
 Humans
 Middle Aged
 Oligonucleotide Array Sequence Analysis
 Papillomaviridae
 Papillomavirus Infections
 Polymerase Chain Reaction
 Uterine Cervical Neoplasms
 Young Adult

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URI

https://hdl.handle.net/20.500.14740/7166

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Scopus: Year 1983-2021

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