Publication: Autologous Fibrin-Base Scaffold for Chondrocytes and Bone Marrow Mesenchymal Stem Cells Implantation: The Development and Comparison to Conventional Fibrin Glue
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Issued Date
2016
Resource Type
Language
eng
File Type
application/pdf
Access Rights
open access
Rights
ผลงานนี้เผยแพร่ภายใต้ สัญญาอนุญาตครีเอทีฟคอมมอนส์แบบ แสดงที่มา-ไม่ใช้เพื่อการค้า-ไม่ดัดแปลง 4.0 (CC BY-NC-ND 4.0)
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
J Med Assoc Thai 2016; 99 (11):99
Suggested Citation
Kasemkijwattana C, Rungsinaporn V, Siripisitsak T, Kongmalai P, Boonprasert R, Charoenthamruksa C, Hongeng S Autologous Fibrin-Base Scaffold for Chondrocytes and Bone Marrow Mesenchymal Stem Cells Implantation: The Development and Comparison to Conventional Fibrin Glue. J Med Assoc Thai 2016; 99 (11):99. Retrieved from: https://hdl.handle.net/20.500.14740/11810
Organization
Abstract
Objective: The authors developed the autologous fibrin-base scaffold for chondrocytes and bone marrow mesenchymal stem
cells (BM-MSCs) implantation and evaluated cells viability in autologous fibrin-base scaffold comparing to commercial fibrin
glue.
Material and Method: The chondrocytes and BM-MSCs were seeded into autologous fibrin-base scaffold and commercial
fibrin glue. The cell viability and proliferation were evaluated at 1 and 7 days. The histology were evaluated with hematoxylineosin
(H&E) staining and cartilaginous matrices formation with Alcian blue, Saffanin-0, Toluidine blue, and Collagen type II staining at 6 weeks. The fixation of the scaffolds was observed.
Results: The chondrocytes and BM-MSCs could not survive in commercial fibrin glue. The chondrocytes and BM-MSCs in
autologous fibrin-base scaffold could proliferate and synthesize the cartilaginous matrices on Alcian blue, Saffanin-0, Toluidine blue, and Collagen type II staining at 6 weeks. The fixation strength is excellent.
Conclusion: The developed autologous fibrin-base scaffold can be used as the scaffold for chondrocytes and BM-MSCs
implantation with potential to implant chondrocytes and BM-MSCs arthroscopically.