Publication: Curcumin and tetrahydrocurcumin restore the impairment of endothelium-dependent vasorelaxation induced by homocysteine thiolactone in rat aortic rings
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Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
18117775
Other identifier(s)
2-s2.0-84858044200
Rights Holder(s)
Scopus
Bibliographic Citation
International Journal of Pharmacology. Vol 8, No.2 (2012), p.128-133
Suggested Citation
Tep-Areenan P., Suksamrarn A. Curcumin and tetrahydrocurcumin restore the impairment of endothelium-dependent vasorelaxation induced by homocysteine thiolactone in rat aortic rings. International Journal of Pharmacology. Vol 8, No.2 (2012), p.128-133. doi:10.3923/ijp.2012.128.133 Retrieved from: https://hdl.handle.net/20.500.14740/7095
Author(s)
Abstract
The aim of the present study was to investigate the effects of curcumin and Terra Hydro Curcumin (THC) on the inhibition of endothelium-dependent vasorelaxation of the isolated rat aorta by Homocysteine Thiolactone (HTL). Carbachol, an endothelium-dependent vasodilator, caused concentration-dependent vasorelaxation in rat aortic rings. Exposure of aortic rings to HTL (0.3 and 1 mM) for 90 min significantly inhibited endothelium-dependent vasorelaxation to carbachol. In addition, contractions induced by methoxamine were significantly reduced after pretreatment with 3 mM HTL. Curcumin (10 and 30 μM) significantly restored carbachol-induced vasorelaxation inhibited by HTL (1 mM). Similar effects were observed after pretreatment of aortic rings with THC (10 and 30 μM). Moreover, HTL-induced impairment of vasorelaxation to carbachol could be blocked by either L-arginine (3 mM), a precursor of nitric oxide or superoxide dismutase (SOD, 200 U mL _1), a scavenger of superoxide anion. These results demonstrate that impairment of endothelium-dependent vasorelaxation induced by HTL is due to a reduction of nitric oxide and the generation of oxygen free radicals. Interestingly, curcumin and THC could restore endothelial dysfunction induced by HTL which may be related to their antioxidant properties. The present study provides pharmacological data to support the hypothesis that curcumin and THC have vasoprotective effects in hyperhomocysteinemia. © 2012 Asian Network for Scientific Information.
Subject(s)
Antioxidant
Arginine
Carbachol
Curcumin
Homocysteine thiolactone
Methoxamine
Nitric oxide
Oxygen radical
Superoxide dismutase
Tetrahydrocurcumin
Unclassified drug
Animal experiment
Animal model
Animal tissue
Antioxidant activity
Aorta constriction
Article
Concentration response
Controlled study
Drug efficacy
Drug structure
Endothelial dysfunction
Long term exposure
Male
Nonhuman
Rat
Structure analysis
Vascular endothelium
Vascular ring
Vasodilatation
Arginine
Carbachol
Curcumin
Homocysteine thiolactone
Methoxamine
Nitric oxide
Oxygen radical
Superoxide dismutase
Tetrahydrocurcumin
Unclassified drug
Animal experiment
Animal model
Animal tissue
Antioxidant activity
Aorta constriction
Article
Concentration response
Controlled study
Drug efficacy
Drug structure
Endothelial dysfunction
Long term exposure
Male
Nonhuman
Rat
Structure analysis
Vascular endothelium
Vascular ring
Vasodilatation