Publication: Antigenicity of hypothetical protein HP33 of Vibrio harveyi Y6 causing scale drop and muscle necrosis disease in Asian sea bass
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Issued Date
2021
Resource Type
File Type
application/pdf
ISSN
10504648
Other identifier(s)
2-s2.0-85097473689
Rights
Srinakharinwirot University
Rights Holder(s)
Scopus
Bibliographic Citation
Fish and Shellfish Immunology. Vol 108, (2021), p.73-79
Suggested Citation
Kwankijudomkul A., Dong H.T., Longyant S., Sithigorngul P., Khunrae P., Rattanarojpong T., Senapin S. Antigenicity of hypothetical protein HP33 of Vibrio harveyi Y6 causing scale drop and muscle necrosis disease in Asian sea bass. Fish and Shellfish Immunology. Vol 108, (2021), p.73-79. doi:10.1016/j.fsi.2020.11.034 Retrieved from: https://hdl.handle.net/20.500.14740/4829
Abstract
A unique strain of Vibrio harveyi is the causative agent of scale drop and muscle necrosis disease (SDMND) in Asian sea bass (Lates calcarifer). This study investigated the protein profiles of SDMND-causing Vibrio harveyi isolates compared to the reference V. harveyi ATCC 14126 strain. A distinct protein band of 33 kDa, namely HP33, found from only V. harveyi SDMND was subjected to analysis by LC-MS/MS and the identified peptide sequences matched to an unknown hypothetical protein. Detection of HP33 coding sequence was investigated at both genomic and transcriptional levels and the results consistently supported the protein analysis. Recombinant HP33 protein was then produced using Escherichia coli system. The rHP33 protein did not cause mortality or visible clinical signs to Asian sea bass. However, the rHP33 protein was able to stimulate antibody response in Asian sea bass as evidenced by Western blotting and agglutination tests. Here, we proposed that rHP33 might be a good protein target for development of subunit vaccine and/or immunostimulant to protect Asian sea bass from SDMND. © 2020 Elsevier Ltd