Publication:
Pathways for movement of fluid and cells from hepatic sinusoids to the portal lymphatic vessels and subcapsular region in rat livers

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Issued Date

2003

Resource Type

Conference Paper

File Type

application/pdf

ISSN

9149465

DOI

10.1679/aohc.66.239

Other identifier(s)

2-s2.0-0141644236

Rights Holder(s)

Scopus

Bibliographic Citation

Archives of Histology and Cytology. Vol 66, No.3 (2003), p.239-252

Suggested Citation

Ohtani Y., Wang B.-J., Poonkhum R., Ohtani O. Pathways for movement of fluid and cells from hepatic sinusoids to the portal lymphatic vessels and subcapsular region in rat livers. Archives of Histology and Cytology. Vol 66, No.3 (2003), p.239-252. doi:10.1679/aohc.66.239 Retrieved from: https://hdl.handle.net/20.500.14740/6572

Title

Pathways for movement of fluid and cells from hepatic sinusoids to the portal lymphatic vessels and subcapsular region in rat livers

Author(s)

Ohtani Y.
 Wang B.-J.
 Poonkhum R.
 Ohtani O.

Abstract

It has long been a mystery how fluid and migrating cells in the hepatic sinusoids reach lymphatic vessels in the portal tract. Here we describe previously-unknown channels that connect the space of Disse with the portal tract in the rat liver. Transmission electron microscopy was performed on livers injected with either horseradish peroxidase (HRP) or lipopolysaccharide, and scanning electron microscopy was carried out on livers macerated with KOH. Transmission electron microscopy revealed the presence of channels with collagen fibers traversing the limiting plate. A tracer study showed that HRP was in the channels as well as along inlet venules. Dendritic cells in the hepatic sinusoids or between hepatocytes of the limiting plate were also observed extending their pseudopodia through the channels in the limiting plate to the interstitial space of the portal tract. Scanning electron microscopy further showed that many channels (1-3 μm in diameter) penetrated through the limiting plate independently of blood vessels and connected the space of Disse with the interstitial space of the portal tract. In addition, the portal tract possessed prelymphatic vessels that were lined with fibroblast-like cells and frequently contained dendritic cells. The initial segment of the portal lymphatic vessels opened to the interstitial tissue space. These results indicate that fluid and dendritic cells in the hepatic sinusoids probably pass through both the space of Disse and the channels traversing the limiting plate, enter the interstitial space of the portal tracts, and finally move from the prelymphatic vessels to the portal lymphatic vessels.

Subject(s)

Collagen fiber
 Horseradish peroxidase
 Lipopolysaccharide
 Potassium hydroxide
 Animal tissue
 Cell function
 Conference paper
 Controlled study
 Dendritic cell
 Fibroblast
 Interstitial fluid
 Interstitium
 Liver sinusoid
 Lymph vessel
 Male
 Membrane channel
 Nonhuman
 Portal vein
 Pseudopodium
 Rat
 Scanning electron microscopy
 Transmission electron microscopy
 Animals
 Dendritic Cells
 Extracellular Space
 Hepatocytes
 Horseradish Peroxidase
 Hydroxides
 Lipopolysaccharides
 Liver
 Lymphatic System
 Male
 Microscopy, Electron
 Microscopy, Electron, Scanning
 Models, Biological
 Potassium Compounds
 Rats
 Rats, Wistar

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URI

https://hdl.handle.net/20.500.14740/6572

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Scopus: Year 1983-2021

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