Publication:
A noradrenergic sensitive endogenous clock is present in the rat pineal gland

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

283835

DOI

10.1159/000327430

Other identifier(s)

2-s2.0-79960932529

Rights Holder(s)

Scopus

Bibliographic Citation

Neuroendocrinology. Vol 94, No.1 (2011), p.75-83

Suggested Citation

Wongchitrat P., Felder-Schmittbuhl M.-P., Govitrapong P., Phansuwan-Pujito P., Simonneaux V. A noradrenergic sensitive endogenous clock is present in the rat pineal gland. Neuroendocrinology. Vol 94, No.1 (2011), p.75-83. doi:10.1159/000327430 Retrieved from: https://hdl.handle.net/20.500.14740/7301

Title

A noradrenergic sensitive endogenous clock is present in the rat pineal gland

Author(s)

Wongchitrat P.
 Felder-Schmittbuhl M.-P.
 Govitrapong P.
 Phansuwan-Pujito P.
 Simonneaux V.

Abstract

The aim of this study was to examine the occurrence of endogenous oscillations of Per1, Per2, Bmal1 and Rev-erbα genes in rat pineal explants and to investigate their regulation by adrenergic ligands. Our results show a significant and sustained rhythm of Per2,Bmal1 and Rev-erbα gene expression for up to 48 h in cultured pineal gland with a pattern similar to that observed in vivo. By contrast, the rhythms of Per1 and Aa-nat, the rate-limiting enzyme for melatonin synthesis, were strongly attenuated after 24 h in culture. Addition of the exogenous adrenergic agonist isoproterenol on cultured pineal glands induced a short-term increase in mRNA levels of Per1 and Aa-nat, but not those of Per2,Bmal1 and Rev-erbα. This study demonstrates that the rat pineal gland hosts a circadian oscillator as evidenced by the sustained, noradrenergic-independent, endogenous oscillations of Per2, Bmal1 and Rev-erbα mRNA levels in cultured tissues. Only expression of Per1 was stimulated by adrenergic ligands suggesting that, in vivo, the adrenergic input could synchronize the pineal clock by acting selectively on Per1. Copyright © 2011 S. Karger AG, Basel.

Subject(s)

Aralkylamine acetyltransferase
 Isoprenaline
 Melatonin
 Nuclear receptor NR1D1
 PER1 protein
 PER2 protein
 Protein BMAL1
 Animal experiment
 Animal tissue
 Article
 Biological rhythm
 Circadian rhythm
 Controlled study
 Gene expression
 Male
 Nonhuman
 Noradrenergic system
 Nucleotide sequence
 Pineal body
 Priority journal
 Rat
 Adrenergic alpha-Agonists
 Adrenergic beta-Agonists
 Adrenergic beta-Antagonists
 Animals
 ARNTL Transcription Factors
 Biological Clocks
 Isoproterenol
 Male
 Models, Animal
 Nuclear Receptor Subfamily 1, Group D, Member 1
 Organ Culture Techniques
 Period Circadian Proteins
 Phenylephrine
 Pineal Gland
 Propranolol
 Rats
 Rats, Wistar

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URI

https://hdl.handle.net/20.500.14740/7301

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Scopus: Year 1983-2021

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