Publication:
Dopamine transporter immunoreactive terminals in the bovine pineal gland

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

3043940

DOI

10.1016/j.neulet.2006.04.066

Other identifier(s)

2-s2.0-33745578684

Rights Holder(s)

Scopus

Bibliographic Citation

Neuroscience Letters. Vol 403, (2006), p.78-83

Suggested Citation

Phansuwan-Pujito P., Boontem P., Chetsawang B., Ebadi M., Govitrapong P. Dopamine transporter immunoreactive terminals in the bovine pineal gland. Neuroscience Letters. Vol 403, (2006), p.78-83. doi:10.1016/j.neulet.2006.04.066 Retrieved from: https://hdl.handle.net/20.500.14740/5578

Title

Dopamine transporter immunoreactive terminals in the bovine pineal gland

Author(s)

Phansuwan-Pujito P.
 Boontem P.
 Chetsawang B.
 Ebadi M.
 Govitrapong P.

Abstract

The dopaminergic system has been proposed to be one of the innervations in controlling the mammalian pineal gland function. The dopamine receptors have been characterized in the pineal and the biphasic effects of dopamine on melatonin production have been demonstrated. Recently, the site of dopamine transporter (DAT), a plasma membrane transport protein of dopaminergic neuron, also has been characterized in the bovine pineal gland. The aim of the present study was to identify the dopaminergic innervation in the bovine pineal gland. The localization and expression of DAT have been performed by using an immunohistochemical method and a reverse transcriptase polymerase chain reaction (RT-PCR) technique. DAT-immunoreactivity was found in the nerve terminals throughout the gland, but not in pinealocytes or neuronal-like cells. Some DAT-immunoreactive nerve fibers were observed along the pineal stalk. DAT mRNA product from RT-PCR was found in the bovine substantia nigra, but not in the pineal gland. The colocalization of DAT with tyrosine hydroxylase (TH) or dopamine beta hydroxylase (DBH) immunoreactivities was observed in nerve terminals. However, no colocalization of DAT with DBH was found in some terminals/fibers. The present results showed the central dopaminergic innervation in the bovine pineal gland distinctively from noradrenergic nerve fibers, and their perikarya origin was located possibly outside of the gland. © 2006 Elsevier Ireland Ltd. All rights reserved.

Subject(s)

Dopamine beta monooxygenase
 Dopamine transporter
 Messenger RNA
 Tyrosine 3 monooxygenase
 Animal tissue
 Article
 Cellular distribution
 Controlled study
 Corpus striatum
 Cow
 Dopaminergic nerve cell
 Immunohistochemistry
 Immunoreactivity
 Nerve cell
 Nerve ending
 Nonhuman
 Perikaryon
 Pineal body
 Pinealocyte
 Priority journal
 Protein expression
 Protein localization
 Reverse transcription polymerase chain reaction
 Substantia nigra
 Tissue distribution
 Animals
 Cattle
 Dopamine beta-Hydroxylase
 Dopamine Plasma Membrane Transport Proteins
 Immunohistochemistry
 Male
 Nerve Endings
 Nerve Fibers
 Pineal Gland
 Presynaptic Terminals
 Reverse Transcriptase Polymerase Chain Reaction
 RNA, Messenger
 Tyrosine 3-Monooxygenase

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URI

https://hdl.handle.net/20.500.14740/5578

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Scopus: Year 1983-2021

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