Publication:
Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity

2
0

Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

17427835

DOI

10.1111/j.1742-7843.2005.pto960112.x

Other identifier(s)

2-s2.0-13844318812

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Basic and Clinical Pharmacology and Toxicology. Vol 96, No.1 (2005), p.80-87

Suggested Citation

Wattanapitayakul S.K., Chularojmontri L., Herunsalee A., Charuchongkolwongse S., Niumsakul S., Bauer J.A. Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity. Basic and Clinical Pharmacology and Toxicology. Vol 96, No.1 (2005), p.80-87. doi:10.1111/j.1742-7843.2005.pto960112.x Retrieved from: https://hdl.handle.net/20.500.14740/6322

Title

Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity

Author(s)

Wattanapitayakul S.K.
 Chularojmontri L.
 Herunsalee A.
 Charuchongkolwongse S.
 Niumsakul S.
 Bauer J.A.

Abstract

Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose-dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/ oxidative stress are a mechanism by which doxorubicin-induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant capacity and cardioprotective effect against doxorubicin-induced cardiotoxicity. The cardiac myoblasts H9c2 were incubated with the antioxidants ascorbic acid, trolox, N-acetylcysteine or selected medicinal plant extracts including; 1) ethanolic extracts from Curcuma longa L-EtOH Phyllanthus emblica L-EtOH, and Piper rostratum Roxb-EtOH; and 2) water extracts from Curcuma longa L-H2O and Morus alba L-H2O. The cardioprotective effects of these extracts were evaluated by crystal violet cytotoxicity assay. IC50s of doxorubicin were compared in the presence or absence of ascorbic acids, trolox, N-acetylcysteine or plant extracts. Morus alba L-H2O showed the highest antioxidant properties evaluated by ferric reducing/antioxidant power assay. Ascorbic acid and N-acetylcysteine had modest effects on the protection of doxorubicin-induced cytotoxicity while trolox showed insignificant protective effect. All plant extracts protected cardiac toxicity at different degrees except that Curcuma longa L-EtOH had no protective effect. Phyllanthus emblica-EtOH (100 μg/ml) showed the highest cardioprotective effect (∼12-fold doxorubicin IC50 increase). The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin.

Subject(s)

Acetylcysteine
 Ascorbic acid
 Crystal violet
 Curcuma longa extract
 Doxorubicin
 Emblica officinalis extract
 Morus alba extract
 Piper rostratum extract
 Plant extract
 Superoxide
 Trolox C
 Unclassified drug
 Animal cell
 Antioxidant activity
 Article
 Cardiotoxicity
 Controlled study
 Cytotoxicity test
 Drug effect
 Drug mechanism
 Drug screening
 Heart protection
 IC 50
 Medicinal plant
 Morus alba
 Myoblast
 Nonhuman
 Oxidative stress
 Piper rostratum
 Priority journal
 Rat
 Animals
 Antibiotics, Antineoplastic
 Antioxidants
 Cardiotonic Agents
 Dose-Response Relationship, Drug
 Doxorubicin
 Drug Evaluation, Preclinical
 Ferric Compounds
 Hela Cells
 Humans
 Myocytes, Cardiac
 Oxidation-Reduction
 Plants, Medicinal
 Rats
 Animalia
 Curcuma longa
 Emblica
 Morus alba
 Phyllanthus emblica
 Viola (angiosperm)

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URI

https://hdl.handle.net/20.500.14740/6322

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Scopus: Year 1983-2021

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