Publication: Antioxidant Activity and Phytochemical Composition of Thai Traditional Cannabis Longevity Recipes: In Vitro Evaluation of the Phontecho Remedy
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Issued Date
2025-12-31
Resource Type
ISSN
26160684
eISSN
26160692
Scopus ID
2-s2.0-105028758596
Journal Title
Tropical Journal of Natural Product Research
Volume
9
Issue
12
Start Page
6166
End Page
6174
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Journal of Natural Product Research Vol.9 No.12 (2025) , 6166-6174
Suggested Citation
Yongram C., Sripan P., Chokchaisiri S., Wonganan O., Meeboonya R., Kamoltham T., Krutchangthong S., Panyatip P. Antioxidant Activity and Phytochemical Composition of Thai Traditional Cannabis Longevity Recipes: In Vitro Evaluation of the Phontecho Remedy. Tropical Journal of Natural Product Research Vol.9 No.12 (2025) , 6166-6174. 6174. doi:10.26538/tjnpr/v9i12.33 Retrieved from: https://hdl.handle.net/20.500.14740/55166
Corresponding Author(s)
Other Contributor(s)
Abstract
Thai traditional medicine has historically incorporated cannabis-based remedies to treat fever, pain, inflammation, insomnia, and to promote longevity. Because antioxidant activity is associated with anti-aging effects, this study investigated the antioxidant potential and phytochemical composition of the Phontecho remedy using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. Phytochemical profiling was performed by high-performance liquid chromatography and gas chromatography–mass spectrometry. Phontecho exhibited notable antioxidant capacity, with a DPPH half-maximal inhibitory concentration (IC₅₀) of 50.33 ± 0.83 µg/mL, an ABTS IC₅₀ of 9.51 ± 0.36 µg/mL, and a FRAP of 382.47 ± 8.61 mmol Fe²⁺/100 g extract. The extract contained high total phenolic content (85.74 ± 2.49 mg gallic acid equivalents/g extract) and moderate total flavonoid content (34.51 ± 0.11 mg quercetin equivalents/g extract). HPLC analysis identified cannabinoids (THCV, Δ⁹-THC, CBDV, CBGA, CBC) and phenolic compounds (gallic acid, quercetin), while GC–MS profiling revealed eugenol, (+)-2-bornanone, γ-sitosterol, and vitamin E. Antioxidant activity correlated with cannabinoid and phenolic acid contents. These phytochemicals provide a mechanistic basis for the antioxidant efficacy of the Phontecho remedy. The findings support its traditional use as a longevitypromoting remedy and highlight potential as a natural antioxidant for pharmacological applications.