Publication:
Development and evaluation of a highly sensitive immunochromatographic strip test using gold nanoparticle for direct detection of Vibrio cholerae O139 in seafood samples

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2012.10.011

Other identifier(s)

2-s2.0-84871652158

Rights Holder(s)

Scopus

Bibliographic Citation

Biosensors and Bioelectronics. Vol 42, No.1 (2013), p.229-235

Suggested Citation

Pengsuk C., Chaivisuthangkura P., Longyant S., Sithigorngul P. Development and evaluation of a highly sensitive immunochromatographic strip test using gold nanoparticle for direct detection of Vibrio cholerae O139 in seafood samples. Biosensors and Bioelectronics. Vol 42, No.1 (2013), p.229-235. doi:10.1016/j.bios.2012.10.011 Retrieved from: https://hdl.handle.net/20.500.14740/6682

Title

Development and evaluation of a highly sensitive immunochromatographic strip test using gold nanoparticle for direct detection of Vibrio cholerae O139 in seafood samples

Author(s)

Pengsuk C.
 Chaivisuthangkura P.
 Longyant S.
 Sithigorngul P.

Abstract

A strip test for the detection of Vibrio cholerae O139 was developed using two monoclonal antibodies (MAbs), namely VC-273 and VC-812, which specifically bind to the lipopolysaccharide and capsular polysaccharide of V. cholerae O139. The MAb VC-273 gold nanoparticle conjugate was sprayed onto a glass fiber pad that was placed adjacent to a sample chamber. MAb VC-812 and the goat anti-mouse immunoglobulin G (GAM) antibody were sprayed onto a nitrocellulose membrane in strips at positions designated as T and C, respectively. The test strips were assessed for their ability to directly detect V. cholerae O139 using samples dispersed in application buffer, and a 100μL aliquot of sample was applied to the sample chamber. The results were observable within 20min after application of the sample. In samples containing V. cholerae O139, the antigen was bound to the colloidal gold-conjugated MAb to form an antibody-antigen complex. This complex was captured by the MAbs at the T test line, resulting in the appearance of a reddish-purple band at the T position. The sensitivity of the test was determined to be 104cfumL-1. Direct detection of V. cholerae O139 in various fresh seafood samples could be accomplished with similar sensitivities. The detection limit was substantially improved to 1cfumL-1 of the original bacterial content after pre-incubation of the sample in alkaline peptone water for 12h. The V. cholerae strip test provides several advantages over other methods, including the speed and simplicity of use because there is no requirement for sophisticated equipment. © 2012 Elsevier B.V.

Subject(s)

Gold Nanoparticles
 Immunochromatography
 Monoclonal antibodies (mAb)
 Strip test
 Vibrio cholerae
 Antigens
 Glass fibers
 Metal nanoparticles
 Monoclonal antibodies
 Nitrocellulose
 Gold
 Glass fiber
 Gold nanoparticle
 Immunoglobulin G antibody
 Lipopolysaccharide
 Monoclonal antibody
 Polysaccharide
 Pyroxylin
 Water
 Antibody production
 Antigen antibody complex
 Antigen binding
 Article
 Bacterium detection
 Colony forming unit
 Conjugation
 Controlled study
 Food analysis
 Immunoaffinity chromatography
 Limit of detection
 Nonhuman
 Process development
 Process optimization
 Sea food
 Sensitivity analysis
 Test strip
 Thermostability
 Vibrio cholerae
 Animals
 Antibodies, Monoclonal
 Food Analysis
 Gold
 Humans
 Metal Nanoparticles
 Mice
 Seafood
 Sensitivity and Specificity
 Vibrio cholerae O139
 Bacteria
 Cellulose Nitrate
 Chromatography
 Glass Fibers
 Gold Compounds
 Bacteria (microorganisms)
 Capra hircus
 Vibrio cholerae

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URI

https://hdl.handle.net/20.500.14740/6682

Collections

Scopus: Year 1983-2021

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