Publication:
Effects of Phikud Navakot extract on vascular reactivity in the isolated rat aorta

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876900461

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S1-S7

Suggested Citation

Nusuetrong P., Sotanaphun U., Tep-Areenan P. Effects of Phikud Navakot extract on vascular reactivity in the isolated rat aorta. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S1-S7. Retrieved from: https://hdl.handle.net/20.500.14740/6899

Title

Effects of Phikud Navakot extract on vascular reactivity in the isolated rat aorta

Author(s)

Nusuetrong P.
 Sotanaphun U.
 Tep-Areenan P.

Abstract

The aim of the present study is to investigate the effect of standardized Phikud Navakot extract (NVKE) on aortic rings from male Sprague Dawley rats. Changes in tension were measured using an isometric force transducer and recorded on the PowerLab recording system. Vasorelaxant effect of NVKE was examined in the presence of indomethacin (10 microM), NG-nitro L-arginine methyl ester (L-NAME, 300 microM), methoxamine (0.1-300 microM), carbachol (1 nanoM-30 microM), or sodium nitroprusside (0.1 nanoM-10 microM). The results showed that NVKE (1-300 microg/mL) caused vasorelaxation in a concentration-dependent manner with a pEC50 value of 4.27 + 0.24 and R max of 67.7 + 13.9%. Pretreatment with indomethacin or L-NAME did not affect NVKE-induced vasorelaxation. However, co-incubation of indomethacin and L-NAME significantly reduced (p < 0.05) vasorelaxation to NVKE (100 microg/mL). Pre-treatment with NVKE significantly decreased (p < 0.05) endothelium-dependent relaxations to carbachol (Rmax = 52.90 + 14.3%), but not to sodium nitroprusside. Moreover, contractions to methoxamine were unaffected after pretreatment with NVKE. The present study suggested that NVKE decreased vasorelaxation to carbachol in the rat aorta, which may exert at least against muscarinic receptors. These findings support the use of Phikud Navakot, a major ingredient of Yahom Navakot, against dizziness and fainting.

Subject(s)

Carbachol
 Indometacin
 Methoxamine
 Nitric oxide synthase
 Nitroprusside sodium
 Phikud Navakot extract
 Plant extract
 Prostaglandin synthase inhibitor
 Unclassified drug
 Animal experiment
 Animal model
 Animal tissue
 Article
 Concentration response
 Controlled study
 Herbal medicine
 Male
 Nonhuman
 Rat
 Thoracic aorta
 Vascular ring
 Vasodilatation
 Analysis of Variance
 Animals
 Aorta, Thoracic
 Carbachol
 Drug Combinations
 Endothelium, Vascular
 Indomethacin
 Male
 Methoxamine
 NG-Nitroarginine Methyl Ester
 Nitroprusside
 Plant Extracts
 Rats
 Rats, Sprague-Dawley
 Transducers
 Vasodilation
 Vasodilator Agents

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URI

https://hdl.handle.net/20.500.14740/6899

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Scopus: Year 1983-2021

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