Publication: High transfection efficiency and low toxicity cationic lipids with aminoglycerol-diamine conjugate
1
0
Issued Date
2009
Resource Type
File Type
application/pdf
ISSN
9680896
Other identifier(s)
2-s2.0-57749199919
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Bioorganic and Medicinal Chemistry. Vol 17, No.1 (2009), p.176-188
Suggested Citation
Yingyongnarongkul B.-E., Radchatawedchakoon W., Krajarng A., Watanapokasin R., Suksamrarn A. High transfection efficiency and low toxicity cationic lipids with aminoglycerol-diamine conjugate. Bioorganic and Medicinal Chemistry. Vol 17, No.1 (2009), p.176-188. doi:10.1016/j.bmc.2008.11.003 Retrieved from: https://hdl.handle.net/20.500.14740/3711
Abstract
The solid phase synthesis of a library of aminoglycerol-diamine conjugate-based transfection agents having urea linkage between diverse length of diamines and various lengths of hydrophobic tails is described. These compounds were characterized and structure-activity relationships were determined for DNA binding and transfection ability when formulated as cationic liposomes. Cationic lipids with short spacer length and short hydrophobic tails bound to DNA and delivered DNA into HEK293 cells more efficient than those with longer ones. Transfection efficiency of some of the cationic liposomes was superior to that of the commercial transfection agents, EffecteneTM, DOTAP and DC-Chol. The lipids 6Ab and 6Bb did not require the helper lipid DOPE to produce high-efficiency transfection of human cells while displaying minimal cytotoxicity. This suggests that these newly described aminoglycerol-based lipids should be very promising in liposome-mediated gene delivery and illustrate the potential of solid phase synthesis method for non-viral vector discovery. © 2008 Elsevier Ltd. All rights reserved.
Subject(s)
1,2 dioleoyl 3 trimethylammoniopropane
Aminoglycerol diamine derivative
Cation
Diamine
Effectene
Lipid
Liposome
Unclassified drug
Virus vector
Article
Cation transport
Conjugation
Controlled study
DNA binding
DNA transfection
Drug cytotoxicity
Drug formulation
Gene targeting
Human
Human cell
Nonviral gene delivery system
Solid phase synthesis
Structure activity relation
Cations
Cell Line
Cross-Linking Reagents
Diamines
DNA
Glycerol
Humans
Lipids
Liposomes
Small Molecule Libraries
Structure-Activity Relationship
Transfection
Aminoglycerol diamine derivative
Cation
Diamine
Effectene
Lipid
Liposome
Unclassified drug
Virus vector
Article
Cation transport
Conjugation
Controlled study
DNA binding
DNA transfection
Drug cytotoxicity
Drug formulation
Gene targeting
Human
Human cell
Nonviral gene delivery system
Solid phase synthesis
Structure activity relation
Cations
Cell Line
Cross-Linking Reagents
Diamines
DNA
Glycerol
Humans
Lipids
Liposomes
Small Molecule Libraries
Structure-Activity Relationship
Transfection