Publication:
Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition of NF-κB activation

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

0258851X

Other identifier(s)

2-s2.0-77955870850

Rights Holder(s)

Scopus

Bibliographic Citation

In Vivo. Vol 24, No.4 (2010), p.501-506

Suggested Citation

Lirdprapamongkol K., Sakurai H., Suzuki S., Koizumi K., Prangsaengtong O., Viriyaroj A., Ruchirawat S., Svasti J., Saiki I. Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition of NF-κB activation. In Vivo. Vol 24, No.4 (2010), p.501-506. Retrieved from: https://hdl.handle.net/20.500.14740/7565

Title

Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition of NF-κB activation

Author(s)

Lirdprapamongkol K.
 Sakurai H.
 Suzuki S.
 Koizumi K.
 Prangsaengtong O.
 Viriyaroj A.
 Ruchirawat S.
 Svasti J.
 Saiki I.

Abstract

Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have previously reported antimetatstatic and antiangiogenic effects of vanillin, a flavoring agent from vanilla. Here we have evaluated the sensitizing effect of vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods: Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection of caspase-3 activation and cleavage of poly (ADP-ribose) polymerase using immunoblot analysis. Effect of treatments on TRAIL signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter assay. Results: Pretreatment of HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway. Vanillin pretreatment inhibited TRAIL-induced phosphorylation of p65 and transcriptional activity of NF-κB. Conclusion: Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.

Subject(s)

Caspase 3
 Immunoglobulin enhancer binding protein
 Mitogen activated protein kinase
 Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
 Phosphatidylinositol 3 kinase
 Synaptotagmin I
 Tumor necrosis factor related apoptosis inducing ligand
 Vanillin
 Apoptosis
 Article
 Cell count
 Cell viability
 Drug mechanism
 Enzyme activation
 HeLa cell
 Human
 Human cell
 Immunoblotting
 Protein cleavage
 Protein phosphorylation
 Uterine cervix cancer
 Antioxidants
 Apoptosis
 Benzaldehydes
 Cell Death
 Cell Survival
 Female
 Genes, Reporter
 Hela Cells
 Humans
 Luciferases
 Neoplasms
 NF-kappa B
 Phosphorylation
 TNF-Related Apoptosis-Inducing Ligand
 Uterine Cervical Neoplasms

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URI

https://hdl.handle.net/20.500.14740/7565

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Scopus: Year 1983-2021

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