Publication: Characterization and Evaluation of Lactobacillus plantarum LC5.2 Isolated from Thai Native Pigs for its Probiotic Potential in Gut Microbiota Modulation and Immune Enhancement
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Issued Date
2025-06-17
Resource Type
eISSN
17388872
Scopus ID
2-s2.0-105009656122
Pubmed ID
40537904
Journal Title
Journal of Microbiology and Biotechnology
Volume
35
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Microbiology and Biotechnology Vol.35 (2025) , e2503028
Suggested Citation
Khongkool K., Taweechotipatr M., Payungporn S., Sawaswong V., Lertworapreecha M. Characterization and Evaluation of Lactobacillus plantarum LC5.2 Isolated from Thai Native Pigs for its Probiotic Potential in Gut Microbiota Modulation and Immune Enhancement. Journal of Microbiology and Biotechnology Vol.35 (2025) , e2503028. doi:10.4014/jmb.2503.03028 Retrieved from: https://hdl.handle.net/20.500.14740/21170
Corresponding Author(s)
Other Contributor(s)
Abstract
Probiotic supplementation, particularly with Lactobacillus species, enhances growth performance, maintains gastrointestinal microbial balance, and prevents infections in livestock. This study isolated Lactobacillus strains from the feces of healthy native pigs in southern Thailand and assessed their probiotic properties and safety through both in vitro and in vivo evaluations. Nine Lactobacillus strains showed probiotic potential, with Lactobacillus plantarum LC5.2 demonstrating the best characteristics. This strain tolerated both acid and bile (100% tolerance) and exhibited strong adhesion properties, including high auto-aggregation (69.74%), cell surface hydrophobicity (77.14%), adhesion to Caco-2 cells (9.31%), and biofilm formation. It also exhibited antibacterial activity, inhibiting EHEC, EPEC, and Salmonella Typhimurium through organic acid production. Co-aggregation with these pathogens ranged from 60.83% to 74.09%. Safety evaluations showed no hemolytic activity, susceptibility to antibiotics, and co-existence with other probiotics. In mice, L. plantarum LC5.2 showed no toxicity, with normal food intake, behavior, and weight gain. No abnormalities were found in the small intestine, colon, liver, or spleen. Mice administered the probiotic had significantly higher intestinal IgA levels. Gut microbiome analysis revealed no notable structural alterations but indicated an increase in beneficial bacteria, including Lactobacillus. These results suggest that L. plantarum LC5.2 demonstrates strong probiotic potential, safety, and benefits for gut health, making it a promising candidate for livestock applications.