Publication:
Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

17355362

DOI

10.4103/1735-5362.297852

Other identifier(s)

2-s2.0-85095132431

Rights Holder(s)

Scopus

Bibliographic Citation

Research in Pharmaceutical Sciences. Vol 15, No.5 (2020), p.491-502

Suggested Citation

Rodboon T., Sirilun S., Okada S., Kariya R., Chontananarth T., Suwannalert P. Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos. Research in Pharmaceutical Sciences. Vol 15, No.5 (2020), p.491-502. doi:10.4103/1735-5362.297852 Retrieved from: https://hdl.handle.net/20.500.14740/4390

Title

Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos

Author(s)

Rodboon T.
 Sirilun S.
 Okada S.
 Kariya R.
 Chontananarth T.
 Suwannalert P.

Abstract

Excessive melanin production caused by overactive tyrosinase (TYR) enzyme results in several dermatological problems. The TYR inhibitor, derived from metabolite changes during fermentation, has been well recognized for pigmentation control. Experimental approach: This study is interested in alternative anti-melanogenic agents from bio-modified Riceberry rice through fermentation. Modified Riceberry rice extract (MRB) was evaluated for its cytotoxicity, melanin content, melanin excretion, and TYR activity in B16 cells. TYR and their melanogenesis-related molecules such as TYR-related proteins-1 and -2, and microphthalmia-associated transcription factor (MITF) were determined. The anti-melanogenic activity and toxicity were also tested using the embryonic zebrafish model. Furthermore, comprehensive genotoxicity testing was verified by cytokinesis-block micronucleus cytome assay. Findings/Results: The study found that non-cytotoxic concentrations of MRB at 20 and 40 mg/mL inhibited melanogenesis and melanin excretion by interfering B16 cell morphology. Cellular TYR enzymatic activity was also suppressed in the treated cells. The mRNA transcription and protein expression levels of TYR and MITF decreased by dose-dependent and time-dependent manners with MRB treatment. In the animal model, MRB was found to be safe and potent for melanogenesis-related TYR inhibition in embryonic zebrafish at 20 and 30 mg/mL. The toxicity of effective doses of MRB showed no genotoxicity and mutagenicity. Conclusion and implications: This study suggests that MRB has anti-melanogenesis potential through TYR and its-related protein inhibitions. MRB is also safe for applications and maybe a promising anti-melanogenic agent for hyperpigmentation control. © 2020 Wolters Kluwer Medknow Publications. All rights reserved.

Subject(s)

Dopachrome tautomerase
 Melanin
 Microphthalmia associated transcription factor
 Modified Riceberry rice extract
 Monophenol monooxygenase
 Oxygenase inhibitor
 Phenylthiourea
 Plant extract
 Tyrosinase related protein 1
 Unclassified drug
 Animal cell
 Animal experiment
 Animal model
 Animal tissue
 Antimelanogenic activity
 Article
 B16 cell line
 Cell differentiation
 Cell structure
 Controlled study
 Cytokinesis
 Cytotoxicity
 Down regulation
 Drug activity
 Embryo
 Enzyme activity
 Enzyme inhibition
 Female
 Fermentation
 Genotoxicity
 Germination
 Hyperpigmentation
 In vivo study
 Male
 Melanogenesis
 Micronucleus test
 MRNA expression level
 Mutagenic activity
 Mutagenicity
 Nonhuman
 Protein expression
 Protein expression level
 Rice
 Riceberry rice
 Saccharomyces cerevisiae
 Zebra fish

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URI

https://hdl.handle.net/20.500.14740/4390

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Scopus: Year 1983-2021

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