Publication:
Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose

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Issued Date

2007

Resource Type

Article

File Type

application/pdf

ISSN

2732289

DOI

10.1007/s12010-007-8022-1

Other identifier(s)

2-s2.0-36949035584

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Applied Biochemistry and Biotechnology. Vol 143, No.2 (2007), p.176-186

Suggested Citation

Watanapokasin R., Sawasjirakij N., Usami S., Kirimura K. Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose. Applied Biochemistry and Biotechnology. Vol 143, No.2 (2007), p.176-186. doi:10.1007/s12010-007-8022-1 Retrieved from: https://hdl.handle.net/20.500.14740/4302

Title

Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose

Author(s)

Watanapokasin R.
 Sawasjirakij N.
 Usami S.
 Kirimura K.

Abstract

The first-stage heterokaryons, obtaining from intergeneric protoplast fusion between Aspergillus niger (Y-b) and Trichoderma viride (M5S51), showed slow growth and mixed morphologies on minimal medium. The fusants were classified into heterokaryon and prototrophic haploid, showing the morphology as that of A. niger. The heterokaryon strains formed conidia with the same nutritional requirements as those of the original auxotrophic mutant strains. After several subcultivations on minimal medium containing d-camphor, some heterokaryon strains formed larger two to seven nuclei/conidium as compared to one nucleus/conidium of the auxotrophic mutant and prototrophic strains, indicating that the new hybrids were generated. Interestingly, three fusant strains AT 11-2-3, AT 11-2-10, and AT 11-2-14 produce 19.2, 6.1, and 10.5 g/l citric acid, respectively, in semisolid culture containing cellulose, whereas A. niger Yang no. 2 could not use carboxymethyl cellulose as the sole carbon source for citric acid production. In addition, the average maximum β-glucosidase and carboxymethylcellulase productions from AT 11-2-3, AT 11-2-10, and AT 11-2-14 were about 16- and 4-folds higher than those of A. niger, respectively. © Humana Press Inc. 2007.

Subject(s)

Aspergillus niger
 Citric acid production
 Intergeneric fusants
 Polyploids
 Trichoderma viride
 Cell culture
 Chromosomes
 Citric acid
 Fungi
 Morphology
 Mutagenesis
 Nutrition
 Viruses
 Biotechnology
 Beta glucosidase
 Camphor
 Carbon
 Carboxymethylcellulose
 Cellulose
 Citric acid
 Deoxyglucose
 Dextro camphor
 Unclassified drug
 Article
 Aspergillus niger
 Conidium
 Controlled study
 Haploidy
 Heterokaryon
 Hybrid
 Micromorphology
 Nonhuman
 Nucleus accumbens
 Polyploidy
 Trichoderma viride
 Aspergillus niger
 Cellulose
 Citric Acid
 Fermentation
 Hybrid Vigor
 Mutation
 Polyploidy
 Trichoderma
 Aspergillus niger
 Dryobalanops
 Trichoderma viride

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URI

https://hdl.handle.net/20.500.14740/4302

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Scopus: Year 1983-2021

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