Publication:
Colorimetric DNA based biosensor combined with loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis by using gold nanoprobe aggregation

0
0

Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

17265479

Other identifier(s)

2-s2.0-84880386914

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Sensors and Transducers. Vol 149, No.2 (2013), p.123-128

Suggested Citation

Kaewphinit T., Santiwatanakul S., Chansiri K. Colorimetric DNA based biosensor combined with loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis by using gold nanoprobe aggregation. Sensors and Transducers. Vol 149, No.2 (2013), p.123-128. Retrieved from: https://hdl.handle.net/20.500.14740/6611

Title

Colorimetric DNA based biosensor combined with loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis by using gold nanoprobe aggregation

Author(s)

Kaewphinit T.
 Santiwatanakul S.
 Chansiri K.

Abstract

Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here, a LAMP method was combined with a gold nanoparticle (AuNPs) probes to detect IS6110 of M. tuberculosis rapidly, and specifically. The reaction amplified DNA and hybridized to thiol modified oligonucleotide probe for 5 min was detected at AuNPs color change 5 min after application. Excluding for the step of DNA extraction, test results could be generated approximately within 1 h. Furthermore, the data indicated that LAMP-AuNPs could detect serial dilution of M. tuberculosis DNA limited as 5 pg of genomic DNA. According to the sensitivity, specificity, less time consuming, low cost and convenience, this technique may prove to be a powerful tool for the early diagnosis of M. tuberculosis. © 2013 IFSA.

Subject(s)

AuNPs
 Constant temperature
 DNA-based biosensors
 Gold nanoparticle
 Loop-mediated isothermal amplifications
 Modified oligonucleotides
 Mycobacterium tuberculosis
 Nanopaticles
 Colorimetry
 Developing countries
 Electric lamps
 Gold
 Isotherms
 Oligonucleotides
 Probes
 DNA

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6611

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By