Publication:
Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1677012

DOI

10.1016/j.mimet.2013.09.013

Other identifier(s)

2-s2.0-84885461259

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Microbiological Methods. Vol 95, No.2 (2013), p.304-311

Suggested Citation

Chaivisuthangkura P., Pengsuk C., Longyant S., Sithigorngul P. Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples. Journal of Microbiological Methods. Vol 95, No.2 (2013), p.304-311. doi:10.1016/j.mimet.2013.09.013 Retrieved from: https://hdl.handle.net/20.500.14740/6518

Title

Evaluation of monoclonal antibody based immunochromatographic strip test for direct detection of Vibrio cholerae O1 contamination in seafood samples

Author(s)

Chaivisuthangkura P.
 Pengsuk C.
 Longyant S.
 Sithigorngul P.

Abstract

A strip test for the detection of Vibrio cholerae O1 was developed using two monoclonal antibodies (MAbs), VC-223 and VC-1226, specific to the lipopolysaccharides of Vibrio cholerae O1 Inaba and Ogawa serovars. The sensitivity of the test was 5×105cfu/mL which was similar to that of dot blot test. The detection limit could be improved to 1cfu/mL of the original bacterial content after pre-incubation of the bacterium in alkaline peptone water (APW) for 12h. Detection of V. cholerae O1 in various fresh seafood samples such as shrimp, blood clam, mussel and oyster could be performed directly with sensitivities ranged from 5×105 to 106cfu/mL. After pre-enrichment of the shrimp sample in APW, the detection sensitivities increased to 102 to 10CFU/mL of the original bacterial content after incubation for 12 and 24h. However, the detection sensitivities were also depending on the content of the other bacteria that might inhibit the growth of V. cholerae during pre-enrichment step. The V. cholerae O1 strip test has advantages in speed, and simplicity in not requiring sophisticated equipment or specialized skills and the sample could be directly examined without requirement for sample processing. © 2013 Elsevier B.V.

Subject(s)

Lipopolysaccharide
 Monoclonal antibody
 Article
 Bacterium contamination
 Bacterium detection
 Clam
 Controlled study
 Food contamination
 Growth inhibition
 Immunoaffinity chromatography
 Limit of detection
 Mussel
 Nonhuman
 Oyster
 Priority journal
 Sea food
 Sensitivity and specificity
 Serotype
 Shrimp
 Thermostability
 Vibrio cholerae
 Immunochromatography
 Monoclonal antibody (MAb)
 Strip test
 Vibrio cholerae
 Antibodies, Bacterial
 Antibodies, Monoclonal
 Colony Count, Microbial
 Food Contamination
 Food Microbiology
 Immunochromatography
 Seafood
 Sensitivity and Specificity
 Vibrio cholerae O1

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URI

https://hdl.handle.net/20.500.14740/6518

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Scopus: Year 1983-2021

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