Publication: Neurite outgrowth and neuroprotective effects of quercetin from caesalpinia mimosoides lamk. on CULTURED P19-derived neurons
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
1741427X
Other identifier(s)
2-s2.0-84880147148
Rights Holder(s)
Scopus
Bibliographic Citation
Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-
Suggested Citation
Tangsaengvit N., Kitphati W., Tadtong S., Bunyapraphatsara N., Nukoolkarn V. Neurite outgrowth and neuroprotective effects of quercetin from caesalpinia mimosoides lamk. on CULTURED P19-derived neurons. Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-. doi:10.1155/2013/838051 Retrieved from: https://hdl.handle.net/20.500.14740/6615
Abstract
Quercetin has been isolated for the first time from ethyl acetate extract of Caesalpinia mimosoides Lamk. C. mimosoides Lamk. (Fabaceae) or Cha rueat (Thai name) is an indigenous plant found in mixed deciduous forest in northern and north-eastern parts of Thailand. Thai rural people consume its young shoots and leaves as a fresh vegetable, as well as it is used for medicinal purposes.The antioxidant capacity in terms of radical scavenging activity of quercetin was determined as IC50 of 3.18 ± 0.07 μg/mL, which was higher than that of Trolox and ascorbic acid (12.54 ± 0.89 and 10.52 ± 0.48 μg/mL, resp.). The suppressive effect of quercetin on both purified and cellular acetylcholinesterase (AChE) enzymes was investigated as IC50 56.84 ± 2.64 and 36.60 ± 2.78 μg/mL, respectively. In order to further investigate the protective ability of quercetin on neuronal cells, P19-derived neurons were used as a neuronal model in this study. As a result, quercetin at a very low dose of 1 nM enhanced survival and induced neurite outgrowth of P19-derived neurons. Furthermore, this flavonoid also possessed significant protection against oxidative stress induced by serum deprivation. Altogether, these findings suggest that quercetin is a multifunctional compound and promising valuable drugs candidate for the treatment of neurodegenerative disease. © 2013 Napat Tangsaengvit et al.
Subject(s)
Acetylcholinesterase
Ascorbic acid
Quercetin
Scavenger
Trolox C
Antioxidant activity
Article
Caesalpinia
Caesalpinia mimosoides
Cell viability
Cholinesterase inhibition
Controlled study
Drug effect
Embryonal carcinoma stem cell
Enzyme purification
Human
Human cell
IC 50
Nerve cell
Nerve cell differentiation
Nerve fiber growth
Neuroblastoma cell
Neuroprotection
Oxidative stress
Priority journal
Ascorbic acid
Quercetin
Scavenger
Trolox C
Antioxidant activity
Article
Caesalpinia
Caesalpinia mimosoides
Cell viability
Cholinesterase inhibition
Controlled study
Drug effect
Embryonal carcinoma stem cell
Enzyme purification
Human
Human cell
IC 50
Nerve cell
Nerve cell differentiation
Nerve fiber growth
Neuroblastoma cell
Neuroprotection
Oxidative stress
Priority journal