Publication: Effects of Inorganic Salts on Enzymatic Saccharification Kinetics of Lignocellulosic Biomass for Biofuel Production
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
Other identifier(s)
2-s2.0-85108911875
Rights Holder(s)
Scopus
Bibliographic Citation
ACM International Conference Proceeding Series. Vol , No. (2021), p.63-67
Suggested Citation
Gundupalli M., Tantayotai P., Rattanaporn K., Pongprayoon W., Phusantisampan T., Sriariyanun M. Effects of Inorganic Salts on Enzymatic Saccharification Kinetics of Lignocellulosic Biomass for Biofuel Production. ACM International Conference Proceeding Series. Vol , No. (2021), p.63-67. doi:10.1145/3458359.3458361 Retrieved from: https://hdl.handle.net/20.500.14740/7854
Abstract
Utilization of agricultural waste, or lignocellulosic biomass, is one of the strategies to reduce the environmental problem caused by combustions after harvesting seasons. Biorefining process to convert lignocellulose to value-Added biochemicals and biofuels has been developed. Inorganic salt pretreatments improve enzymatic saccharification efficiency. However, salt residues inhibit activities of cellulase enzyme. In this work, the inhibitory effects of two types of inorganic salts, NaCl and KCl, on commercial cellulase enzyme, Accellerase 1500, were studies using the Michaelis-Menten model. The kinetic properties, in terms of Km and Vm, on three types of cellulose substrates, including CMC, Avicel, and sugarcane bagasse, were calculated based on Lineweaver-Burk plot. The obtained kinetic values showed that Vm of Accellerase 1500 reduced to 58.21% and 81.25% with the presence of 0.5 M KCl when using CMC and Avicel as substrates, respectively. On the other hand, the Km values of cellulases were also reduced with the presence of NaCl and KCl, suggesting the accessibility of enzymes to substrates. Additionally, sugar yields obtained from sugarcane baggase as substrates for biofuel production were also increased with the presence of 1M NaCl and 1M KCl. © 2021 ACM.
Subject(s)
Agricultural robots
Agricultural wastes
Biofuels
Biomass
Cellulose derivatives
Cellulosic ethanol
Enzymes
Kinetics
Potassium compounds
Saccharification
Sodium chloride
Substrates
Sugar industry
Biofuel production
Cellulose substrates
Environmental problems
Enzymatic saccharification
Kinetic properties
Lineweaver-Burk plots
Michaelis-Menten model
Sugar-cane bagasse
Lignocellulosic biomass
Bagasse
Biomass
Carboxymethyl Cellulose
Cellulase
Inorganic Salts
Production
Saccharification
Substrates
Agricultural wastes
Biofuels
Biomass
Cellulose derivatives
Cellulosic ethanol
Enzymes
Kinetics
Potassium compounds
Saccharification
Sodium chloride
Substrates
Sugar industry
Biofuel production
Cellulose substrates
Environmental problems
Enzymatic saccharification
Kinetic properties
Lineweaver-Burk plots
Michaelis-Menten model
Sugar-cane bagasse
Lignocellulosic biomass
Bagasse
Biomass
Carboxymethyl Cellulose
Cellulase
Inorganic Salts
Production
Saccharification
Substrates