Publication:
Stability, Cytotoxicity, and Retinal Pigment Epithelial Cell Binding of Hyaluronic Acid-Coated PLGA Nanoparticles Encapsulating Lutein

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

15309932

DOI

10.1208/s12249-018-1256-0

Other identifier(s)

2-s2.0-85058760535

Rights Holder(s)

Scopus

Bibliographic Citation

AAPS PharmSciTech. Vol 20, No.1 (2019)

Suggested Citation

Chittasupho C., Posritong P., Ariyawong P. Stability, Cytotoxicity, and Retinal Pigment Epithelial Cell Binding of Hyaluronic Acid-Coated PLGA Nanoparticles Encapsulating Lutein. AAPS PharmSciTech. Vol 20, No.1 (2019). doi:10.1208/s12249-018-1256-0 Retrieved from: https://hdl.handle.net/20.500.14740/5664

Title

Stability, Cytotoxicity, and Retinal Pigment Epithelial Cell Binding of Hyaluronic Acid-Coated PLGA Nanoparticles Encapsulating Lutein

Author(s)

Chittasupho C.
 Posritong P.
 Ariyawong P.

Abstract

The application of lutein was limited due to water insolubility and susceptible to heat and light degradation. In this study, hyaluronic acid (HA)-coated PLGA nanoparticles encapsulating lutein were fabricated by a solvent displacement method to improve the physicochemical properties and the stability of lutein. A biphasic release profile of lutein was observed, following zero-order release kinetics. The physical stability of lutein stored at 4°C, 30°C, and 40°C for 30 days was enhanced when lutein was encapsulated in the nanoparticles. The degradation of lutein in PLGA NPs coated with HA was fitted to a second-order kinetic model. The rate constant increased with increasing storage temperature. The activation energy of lutein-NPs was 63.26 kJ/mol. The half-lives of lutein in PLGA-NPs were about 49, 4, and 2 days at a storage temperature of 4°C, 30°C, and 40°C, respectively. The results suggested that lutein-NPs should be stored at 4°C to prevent physical and chemical degradation. The photodegradation of lutein in NPs followed a second-order kinetic model. The rate constant was 0.0155 mg -1  ml day -1 . Cell viability study revealed that HA-coated PLGA nanoparticles encapsulating lutein did not show toxicity against retinal pigment epithelial cells (ARPE-19). The NPs bound ARPE-19 cells in a time- and a dose-dependent manner. The binding efficiency of lutein-NPs decreased at higher concentrations, suggesting that the NPs might reach binding saturation capacity. In conclusion, HA-coated PLGA nanoparticles could be used to deliver lutein and improved physicochemical property of lutein. [Figure not available: see fulltext.]. © 2018, American Association of Pharmaceutical Scientists.

Subject(s)

Hyaluronic acid
 Nanoparticle
 Polyglactin
 Xanthophyll
 Hyaluronic acid
 Nanoparticle
 Xanthophyll
 ARPE-19 cell line
 Article
 Cell viability
 Controlled study
 Dispersity
 Drug binding
 Drug cytotoxicity
 Drug degradation
 Drug release
 Drug solubility
 Drug stability
 In vitro study
 Nanoencapsulation
 Particle size
 Photodegradation
 Priority journal
 Retina pigment cell
 Retinal pigment epithelium
 Storage temperature
 Zeta potential
 Cell culture
 Cell survival
 Chemistry
 Drug effect
 Drug stability
 Epithelium cell
 Human
 Metabolism
 Retinal pigment epithelium
 Cell Survival
 Cells, Cultured
 Drug Stability
 Epithelial Cells
 Humans
 Hyaluronic Acid
 Lutein
 Nanoparticles
 Polylactic Acid-Polyglycolic Acid Copolymer
 Retinal Pigment Epithelium

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URI

https://hdl.handle.net/20.500.14740/5664

Collections

Scopus: Year 1983-2021

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