Publication: Evaluation of a monoclonal antibody-based dot-blot ELISA for detection of leptospira spp inbovine urine samples
1
0
Issued Date
2005
Resource Type
File Type
application/pdf
ISSN
29645
Other identifier(s)
2-s2.0-20444463125
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
American Journal of Veterinary Research. Vol 66, No.5 (2005), p.762-766
Suggested Citation
Suwimonteerabutr J., Chaicumpa W., Saengjaruk P., Tapchaisri P., Chongsa-Nguan M., Kalambaheti T., Ramasoota P., Sakolvaree Y., Virakul P. Evaluation of a monoclonal antibody-based dot-blot ELISA for detection of leptospira spp inbovine urine samples. American Journal of Veterinary Research. Vol 66, No.5 (2005), p.762-766. doi:10.2460/ajvr.2005.66.762 Retrieved from: https://hdl.handle.net/20.500.14740/6149
Abstract
Objective - To evaluate the efficacy of a novel monoclonal antibody (MAb)-based dot-blot ELISA for detection of Leptospira antigens in urine samples of cattle. Sample population - Blood and urine samples of 45 test cattle from 5 farms in Chonburi province and 20 control cattle from 2 farms in Khon Kaen province in Thailand. Procedure - Blood and urine samples were assayed (microscopic agglutination test and urine antigen test) for Leptospira infection by use of an MAb-based dot-blot ELISA, and results for the ELISA were compared with those for dark-field microscopy (DFM), microbial culture, and a polymerase chain reaction (PCR) assay. Results - All urine samples with positive results for DFM, microbial culture, PCR assay, or > 1 of these tests also had positive results when tested by use of the MAb-based dot-blot ELISA, except for 1 sample that had positive results only for the PCR assay. Detection limits of the dot-blot ELISA were 103 leptospires/mL of urine and 9.3 ng of Leptospira homogenate. Comparison revealed that the diagnostic sensitivity, specificity, efficacy (accuracy), positive predictive value, and negative predictive value for the ELISA were in agreement with results for DFM (100%, 72.72%, 80%, 57.14%, and 100%, respectively), microbial culture (100%, 61.54%, 66.62%, 28.57%, and 100%, respectively), and PCR assay (95.45%, 100%, 91.77%, 100%, and 95.83%, respectively). Conclusions and clinical relevance - The MAb-based dot-blot ELISA is suitable as a tool for detecting leptospires in urine samples of cattle.
Subject(s)
Bacterial antigen
Monoclonal antibody
Agglutination test
Antigen detection
Article
Bacterium culture
Bacterium detection
Cattle
Controlled study
Diagnostic accuracy
Diagnostic value
Enzyme linked immunosorbent assay
Female
Intermethod comparison
Leptospira
Leptospirosis
Male
Microscopy
Nonhuman
Polymerase chain reaction
Prediction
Sensitivity and specificity
Thailand
Urinalysis
Animals
Antibodies, Monoclonal
Cattle
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
Female
Immunoblotting
Leptospira
Leptospirosis
Male
Reproducibility of Results
Sensitivity and Specificity
Bos taurus
Leptospira
Monoclonal antibody
Agglutination test
Antigen detection
Article
Bacterium culture
Bacterium detection
Cattle
Controlled study
Diagnostic accuracy
Diagnostic value
Enzyme linked immunosorbent assay
Female
Intermethod comparison
Leptospira
Leptospirosis
Male
Microscopy
Nonhuman
Polymerase chain reaction
Prediction
Sensitivity and specificity
Thailand
Urinalysis
Animals
Antibodies, Monoclonal
Cattle
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
Female
Immunoblotting
Leptospira
Leptospirosis
Male
Reproducibility of Results
Sensitivity and Specificity
Bos taurus
Leptospira