Publication: Enhancement of lymphangiogenesis in vitro via the regulations of HIF-1 α expression and nuclear translocation by deoxyshikonin
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
1741427X
Other identifier(s)
2-s2.0-84877964031
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-
Suggested Citation
Prangsaengtong O., Park J.Y., Inujima A., Igarashi Y., Shibahara N., Koizumi K. Enhancement of lymphangiogenesis in vitro via the regulations of HIF-1 α expression and nuclear translocation by deoxyshikonin. Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-. doi:10.1155/2013/148297 Retrieved from: https://hdl.handle.net/20.500.14740/6655
Abstract
The objectives of this study were to determine the effects of deoxyshikonin on lymphangiogenesis. Deoxyshikonin enhanced the ability of human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) to undergo time-dependent in vitro cord formation. Interestingly, an opposite result was observed in cells treated with shikonin. The increased cord formation ability following deoxyshikonin treatment correlated with increased VEGF-C mRNA expression to higher levels than seen for VEGF-A and VEGF-D mRNA expression. We also found that deoxyshikonin regulated cord formation of HMVEC-dLy by increasing the HIF-1α mRNA level, HIF-1α protein level, and the accumulation of HIF-1α in the nucleus. Knockdown of the HIF-1α gene by transfection with siHIF-1α decreased VEGF-C mRNA expression and cord formation ability in HMVEC-dLy. Deoxyshikonin treatment could not recover VEGF-C mRNA expression and cord formation ability in HIF-1α knockdown cells. This indicated that deoxyshikonin induction of VEGF-C mRNA expression and cord formation in HMVEC-dLy on Matrigel occurred mainly via HIF-1α regulation. We also found that deoxyshikonin promoted wound healing in vitro by the induction of HMVEC-dLy migration into the wound gap. This study describes a new effect of deoxyshikonin, namely, the promotion of cord formation by human endothelial cells via the regulation of HIF-1α. The findings suggest that deoxyshikonin may be a new drug candidate for wound healing and treatment of lymphatic diseases. © 2013 Orawin Prangsaengtong et al.
Subject(s)
Deoxyshikonin
Hypoxia inducible factor 1alpha
Messenger RNA
Shikonin derivative
Unclassified drug
Vasculotropin C
Article
Cell function
Controlled study
Drug mechanism
Endothelium cell
Gene expression regulation
Gene silencing
Gene translocation
Genetic transfection
Human
Human cell
In vitro study
Lymphangiogenesis
Priority journal
Protein expression
Hypoxia inducible factor 1alpha
Messenger RNA
Shikonin derivative
Unclassified drug
Vasculotropin C
Article
Cell function
Controlled study
Drug mechanism
Endothelium cell
Gene expression regulation
Gene silencing
Gene translocation
Genetic transfection
Human
Human cell
In vitro study
Lymphangiogenesis
Priority journal
Protein expression